{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:12:40Z","timestamp":1790212360614,"version":"4.1.0"},"reference-count":79,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Yersinia pestis<\/jats:italic>\n                    , the causative agent of plague, autoaggregates within a few minutes of cessation of shaking when grown at 28\u00b0C. To identify the autoaggregation factor of\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    , we performed\n                    <jats:italic>mariner<\/jats:italic>\n                    -based transposon mutagenesis. Autoaggregation-defective mutants from three different pools were identified, each with a transposon insertion at a different position within the gene encoding phosphoglucomutase (\n                    <jats:italic>pgmA<\/jats:italic>\n                    ;\n                    <jats:italic>y1258<\/jats:italic>\n                    ). Targeted deletion of\n                    <jats:italic>pgmA<\/jats:italic>\n                    in\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    KIM5 also resulted in loss of autoaggregation. Given the previously defined role for phosphoglucomutase in antimicrobial peptide resistance in other organisms, we tested the KIM5 \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant for antimicrobial peptide sensitivity. The \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant displayed &gt;1,000-fold increased sensitivity to polymyxin B compared to the parental\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    strain, KIM5. This sensitivity is not due to changes in lipopolysaccharide (LPS) since the LPSs from both\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    KIM5 and the \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant are identical based on a comparison of their structures by mass spectrometry (MS), tandem MS, and nuclear magnetic resonance analyses. Furthermore, the ability of polymyxin B to neutralize LPS toxicity was identical for LPS purified from both KIM5 and the \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant. Our results indicate that increased polymyxin B sensitivity of the \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant is due to changes in surface structures other than LPS. Experiments with mice via the intravenous and intranasal routes did not demonstrate any virulence defect for the \u0394\n                    <jats:italic>pgmA<\/jats:italic>\n                    mutant, nor was flea colonization or blockage affected. Our findings suggest that the activity of PgmA results in modification and\/or elaboration of a surface component of\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    responsible for autoaggregation and polymyxin B resistance.\n                  <\/jats:p>","DOI":"10.1128\/iai.00997-09","type":"journal-article","created":{"date-parts":[[2009,12,22]],"date-time":"2009-12-22T21:10:09Z","timestamp":1261516209000},"page":"1163-1175","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Phosphoglucomutase of\n                    <i>Yersinia pestis<\/i>\n                    Is Required for Autoaggregation and Polymyxin B Resistance"],"prefix":"10.1128","volume":"78","author":[{"given":"Suleyman","family":"Felek","sequence":"first","affiliation":[{"name":"Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Muszyn\u0301ski","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell W.","family":"Carlson","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany M.","family":"Tsang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan School of Medicine, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Joseph","family":"Hinnebusch","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Krukonis","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan"},{"name":"Department of Microbiology and Immunology, University of Michigan School of Medicine, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1202"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2146\/ajhp060473"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29256-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.17.5298-5307.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.7.3033-3040.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.58.2.145-161.1994"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4528-4535.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-4-1115"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6935-6944.2005"},{"key":"e_1_3_2_11_2","first-page":"2691","year":"2005","unstructured":"Butler, T., and D. T. Dennis. 2005. Yersinia species, including plague, p. 2691-2700. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious diseases, 6th ed. Churchill Livingstone, Philadelphia, PA.","journal-title":"Principles and practice of infectious diseases"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.4.2172-2174.1990"},{"key":"e_1_3_2_13_2","unstructured":"In the wake of the plague. 2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(88)84149-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17061133.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.12.3500-3507.1994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/417243a"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(96)00061-X"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46733-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00913-08"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/010918-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01446.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2646710"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5088-5097.2001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00601.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.10.4166-4169.1995"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2829-2837.2001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.7040-7045.1997"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.11939"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0325z.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.09.006"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05100.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00757.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1086\/314456"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5273.367"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.20.12296"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/422695"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.42.1.163-169.1983"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0582-z"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.4049\/jimmunol.151.5.2742","volume":"151","year":"1993","unstructured":"Kirikae, T., F. U. Schade, F. Kirikae, E. T. Rietschel, and D. C. Morrison. 1993. Isolation of a macrophage-like cell line defective in binding of lipopolysaccharide. Influence of serum and lipopolysaccharide chain length on macrophage activation. J. Immunol.151:2742-2752.","journal-title":"J. Immunol."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048430f"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/005694-0"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-38-2-157"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.4.430-432.1980"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.39-45.2005"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.8.2569-2577.1990"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.18.5847-5851.1994"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3068-3075.2003"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.11.3706-3710.1990"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90007-X"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2618"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3419-3425.2000"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.35"},{"key":"e_1_3_2_55_2","first-page":"131","volume":"9","year":"1953","unstructured":"Pollitzer, R. 1953. Plague studies. IX. Epidemiology. Bull. World Health Organ.9:131-170.","journal-title":"IX. Epidemiology. Bull. World Health Organ."},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04059.x"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00705-0"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/334522a0"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1645"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(03)00305-7"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.158.3.1033-1036.1984"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00198.x"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.417-425.1991"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.4.1200-1210.1989"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-49-2-127"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02372-0"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.10.5716-5723.2000"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01952-06"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.139.2.664-667.1979"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4673-4680.2000"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413900200"},{"key":"e_1_3_2_72_2","first-page":"301","volume":"79","year":"2004","unstructured":"World Health Organization. 2004. Human plague in 2002 and 2003. Wkly. Epidemiol. Rec.79:301-306.","journal-title":"Wkly. Epidemiol. Rec."},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3511719.x"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687860600927626"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(86)18062-1"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100127597"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm357"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.06.053"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)78105-8"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)92885-1"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00997-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00997-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T20:33:56Z","timestamp":1685306036000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00997-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":79,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1128\/IAI.00997-09"],"URL":"https:\/\/doi.org\/10.1128\/iai.00997-09","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.719347191.793518906","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"2009-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}