{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:25:19Z","timestamp":1766157919057,"version":"3.37.3"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,1,25]],"date-time":"2016-01-25T00:00:00Z","timestamp":1453680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Werner Siemens Foundation"},{"DOI":"10.13039\/501100000781","name":"EC | European Research Council","doi-asserted-by":"publisher","award":["2011-293605-CAPCAN"],"award-info":[{"award-number":["2011-293605-CAPCAN"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["3100A0-128659"],"award-info":[{"award-number":["3100A0-128659"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Capnocytophaga canimorsus<\/jats:named-content>\n            , a commensal bacterium of dog's mouth flora causing severe infections in humans after dog bites or scratches, has a lipopolysaccharide (LPS) (endotoxin) with low-inflammatory lipid A. In particular, it contains a phosphoethanolamine (\n            <jats:italic>P<\/jats:italic>\n            -Etn) instead of a free phosphate group at the C-1 position of the lipid A backbone, usually present in highly toxic enterobacterial Gram-negative lipid A. Here we show that the\n            <jats:named-content content-type=\"genus-species\">C. canimorsus<\/jats:named-content>\n            genome comprises a single operon encoding a lipid A 1-phosphatase (LpxE) and a lipid A 1\n            <jats:italic>P<\/jats:italic>\n            -Etn transferase (EptA). This suggests that lipid A is modified during biosynthesis after completing acylation of the backbone by removal of the 1-phosphate and subsequent addition of an\n            <jats:italic>P<\/jats:italic>\n            -Etn group. As endotoxicity of lipid A is known to depend largely on the degree of unsubstituted or unmodified phosphate residues, deletion of\n            <jats:italic>lpxE<\/jats:italic>\n            or\n            <jats:italic>eptA<\/jats:italic>\n            led to mutants lacking the\n            <jats:italic>P<\/jats:italic>\n            -Etn group, with consequently increased endotoxicity and decreased resistance to cationic antimicrobial peptides (CAMP). Consistent with the proposed sequential biosynthetic mechanism, the endotoxicity and CAMP resistance of a double deletion mutant of\n            <jats:italic>lpxE-eptA<\/jats:italic>\n            was similar to that of a single\n            <jats:italic>lpxE<\/jats:italic>\n            mutant. Finally, the proposed enzymatic activities of LpxE and EptA based on sequence similarity could be successfully validated by mass spectrometry (MS)-based analysis of lipid A isolated from the corresponding deletion mutant strains.\n          <\/jats:p>","DOI":"10.1128\/iai.01006-15","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T15:05:36Z","timestamp":1449587136000},"page":"550-561","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Modification of the 1-Phosphate Group during Biosynthesis of Capnocytophaga canimorsus Lipid A"],"prefix":"10.1128","volume":"84","author":[{"given":"Francesco","family":"Renzi","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Z\u00e4hringer","sequence":"additional","affiliation":[{"name":"Division of Immunochemistry, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Borstel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney E.","family":"Chandler","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert K.","family":"Ernst","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy R.","family":"Cornelis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Namur, Namur, Belgium"},{"name":"Biozentrum der Universit\u00e4t Basel, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J.","family":"Ittig","sequence":"additional","affiliation":[{"name":"Biozentrum der Universit\u00e4t Basel, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_3_2_2","DOI":"10.1111\/j.1462-5822.2009.01349.x"},{"doi-asserted-by":"publisher","key":"e_1_3_3_3_2","DOI":"10.1177\/154405910508400702"},{"doi-asserted-by":"publisher","key":"e_1_3_3_4_2","DOI":"10.1038\/ni777"},{"doi-asserted-by":"publisher","key":"e_1_3_3_5_2","DOI":"10.1126\/science.1138963"},{"doi-asserted-by":"publisher","key":"e_1_3_3_6_2","DOI":"10.1073\/pnas.92.16.7352"},{"doi-asserted-by":"publisher","key":"e_1_3_3_7_2","DOI":"10.1074\/jbc.M409078200"},{"doi-asserted-by":"publisher","key":"e_1_3_3_8_2","DOI":"10.1074\/jbc.M600435200"},{"doi-asserted-by":"publisher","key":"e_1_3_3_9_2","DOI":"10.1128\/IAI.00900-10"},{"doi-asserted-by":"publisher","key":"e_1_3_3_10_2","DOI":"10.1111\/j.1365-2958.2010.07150.x"},{"doi-asserted-by":"publisher","key":"e_1_3_3_11_2","DOI":"10.1016\/j.bbalip.2010.02.001"},{"doi-asserted-by":"publisher","key":"e_1_3_3_12_2","DOI":"10.1073\/pnas.0611606104"},{"doi-asserted-by":"publisher","key":"e_1_3_3_13_2","DOI":"10.1371\/journal.ppat.1002454"},{"doi-asserted-by":"publisher","key":"e_1_3_3_14_2","DOI":"10.1128\/IAI.06109-11"},{"doi-asserted-by":"publisher","key":"e_1_3_3_15_2","DOI":"10.1021\/bi101253s"},{"doi-asserted-by":"publisher","key":"e_1_3_3_16_2","DOI":"10.1074\/jbc.M406480200"},{"doi-asserted-by":"publisher","key":"e_1_3_3_17_2","DOI":"10.1128\/JB.00146-06"},{"doi-asserted-by":"publisher","key":"e_1_3_3_18_2","DOI":"10.1128\/JB.185.11.3270-3277.2003"},{"doi-asserted-by":"publisher","key":"e_1_3_3_19_2","DOI":"10.1099\/mic.0.28692-0"},{"doi-asserted-by":"publisher","key":"e_1_3_3_20_2","DOI":"10.1371\/journal.ppat.1002667"},{"doi-asserted-by":"publisher","key":"e_1_3_3_21_2","DOI":"10.1093\/ajcp\/65.4.564"},{"doi-asserted-by":"publisher","key":"e_1_3_3_22_2","DOI":"10.1128\/jcm.27.2.231-235.1989"},{"doi-asserted-by":"publisher","key":"e_1_3_3_23_2","DOI":"10.1007\/s10096-015-2360-7"},{"doi-asserted-by":"publisher","key":"e_1_3_3_24_2","DOI":"10.1007\/BF00144007"},{"doi-asserted-by":"publisher","key":"e_1_3_3_25_2","DOI":"10.1093\/clinids\/23.1.71"},{"doi-asserted-by":"publisher","key":"e_1_3_3_26_2","DOI":"10.1128\/jcm.7.2.223-231.1978"},{"doi-asserted-by":"publisher","key":"e_1_3_3_27_2","DOI":"10.1016\/S0163-4453(98)93918-4"},{"doi-asserted-by":"publisher","key":"e_1_3_3_28_2","DOI":"10.1016\/j.micinf.2009.02.005"},{"doi-asserted-by":"publisher","key":"e_1_3_3_29_2","DOI":"10.1128\/JB.05853-11"},{"doi-asserted-by":"publisher","key":"e_1_3_3_30_2","DOI":"10.1146\/annurev.bi.59.070190.001021"},{"doi-asserted-by":"publisher","key":"e_1_3_3_31_2","DOI":"10.1086\/510243"},{"doi-asserted-by":"publisher","key":"e_1_3_3_32_2","DOI":"10.1128\/AEM.01218-08"},{"key":"e_1_3_3_33_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard","author":"NCCLS\/CLSI","year":"2003","unstructured":"NCCLS\/CLSI. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. National Committee for Clinical Laboratory Standards\/Clinical Laboratory and Standards Institute, Wayne, PA."},{"doi-asserted-by":"publisher","key":"e_1_3_3_34_2","DOI":"10.1093\/nar\/25.17.3389"},{"doi-asserted-by":"publisher","key":"e_1_3_3_35_2","DOI":"10.1074\/jbc.M114.571489"},{"doi-asserted-by":"publisher","key":"e_1_3_3_36_2","DOI":"10.1128\/IAI.01324-08"},{"doi-asserted-by":"publisher","key":"e_1_3_3_37_2","DOI":"10.1194\/jlr.D500014-JLR200"},{"doi-asserted-by":"publisher","key":"e_1_3_3_38_2","DOI":"10.1096\/fasebj.8.2.8119492"},{"key":"e_1_3_3_39_2","first-page":"21202","article-title":"A phosphoethanolamine transferase specific for the outer 3-deoxy-d-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide","volume":"280","author":"Reynolds CM","year":"2005","unstructured":"Reynolds CM, Kalb SR, Cotter RJ, Raetz CR. 2005. 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