{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T07:54:30Z","timestamp":1789372470712,"version":"build-2803163510"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The progression of\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            infection in macrophages is controlled by the\n            <jats:italic>Lgn1<\/jats:italic>\n            gene locus, which expresses the nonpermissive phenotype in cells from BALB\/c mice but the permissive phenotype in cells from A\/J mice. Activation of dendritic cells and macrophages by\n            <jats:italic>L. pneumophila<\/jats:italic>\n            is mediated by the pathogen recognition receptor Toll-like receptor 2 (TLR2); furthermore,\n            <jats:italic>Legionella<\/jats:italic>\n            induces innate and adaptive immune cytokines by the MyD88-dependent pathway. TLR9 is coupled to MyD88 and mediates the production of interleukin-12 (IL-12) in dendritic cells infected with other facultatively intracellular pathogens. In the current study,\n            <jats:italic>L. pneumophila<\/jats:italic>\n            growth in dendritic cells from BALB\/c and A\/J mice was examined along with the role of TLR9 in the induction of IL-12 in these cells. Dendritic cells from both strains were nonpermissive for\n            <jats:italic>L. pneumophila<\/jats:italic>\n            intracellular growth, suggesting that the products of the\n            <jats:italic>Lgn1<\/jats:italic>\n            gene locus that control intracellular growth in macrophages do not control the growth of\n            <jats:italic>Legionella<\/jats:italic>\n            in dendritic cells. In addition, chloroquine treatment suppressed IL-12 p40 production in response to\n            <jats:italic>Legionella<\/jats:italic>\n            treatment in dendritic cells and macrophages from BALB\/c and A\/J mice. Furthermore, the TLR9 inhibitor ODN2088 suppressed the\n            <jats:italic>Legionella<\/jats:italic>\n            -induced IL-12 production in dendritic cells from both mouse strains. These results suggest that\n            <jats:italic>L. pneumophila<\/jats:italic>\n            is similar to other intracellular bacteria in that it stimulates the production of immune-transitioning cytokines, such as IL-12, through activation of TLR9 and that this receptor provides a common mechanism for sensing these types of microbes and inducing innate and adaptive immunity.\n          <\/jats:p>","DOI":"10.1128\/iai.01011-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"146-151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Role of Toll-Like Receptor 9 in\n            <i>Legionella pneumophila<\/i>\n            -Induced Interleukin-12 p40 Production in Bone Marrow-Derived Dendritic Cells and Macrophages from Permissive and Nonpermissive Mice"],"prefix":"10.1128","volume":"75","author":[{"given":"Cathy A.","family":"Newton","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabella","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond H.","family":"Widen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida"},{"name":"Tampa General Hospital, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Friedman","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas W.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5339-5349.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.352-361.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/90609"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.950-958.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02049-05"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051782"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519050110020401"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115301"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.1.55"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.4102"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6221-6229.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1065"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.08.016"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.08.015"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.05.007"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00212"},{"key":"e_1_3_2_18_2","first-page":"1118","volume":"17","year":"1989","unstructured":"Harley, V. 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