{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T07:05:38Z","timestamp":1763967938741},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Haemophilus ducreyi<\/jats:italic>\n            is a gram-negative bacterium that is the causative agent of chancroid. Strain 35000HP has been well characterized and is representative of the majority of\n            <jats:italic>H. ducreyi<\/jats:italic>\n            strains. Strain 35000HP produces a lipooligosaccharide (LOS) that contains\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>glycero<\/jats:italic>\n            -\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>manno<\/jats:italic>\n            -heptose in the main oligosaccharide chain extension; the\n            <jats:italic>lbgB<\/jats:italic>\n            gene has been shown to encode the\n            <jats:sc>dd<\/jats:sc>\n            -heptosyltransferase. The\n            <jats:italic>lbgB<\/jats:italic>\n            gene is found in a gene cluster together with the\n            <jats:italic>lbgA<\/jats:italic>\n            gene, which encodes for the galactosyltransferase I. These two genes are flanked by two housekeeping genes,\n            <jats:italic>rpmE<\/jats:italic>\n            and\n            <jats:italic>xthA<\/jats:italic>\n            , encoding the ribosomal protein L31 and the exonuclease III, respectively. Recently, a second group of\n            <jats:italic>H. ducreyi<\/jats:italic>\n            strains have been identified. Strain 33921, a representative of the class II strains, produces an LOS that lacks\n            <jats:sc>dd<\/jats:sc>\n            -heptose in the oligosaccharide portion of its LOS. To better understand the biosynthesis of the\n            <jats:sc>dd<\/jats:sc>\n            -heptose-deficient 33921 LOS, we cloned and sequenced the corresponding\n            <jats:italic>lbgAB<\/jats:italic>\n            genomic region from strain 33921. Similar to strain 35000HP, the 33921 genome contains\n            <jats:italic>xthA<\/jats:italic>\n            and\n            <jats:italic>rpmE<\/jats:italic>\n            . However, between these two genes we identified genes encoding two putative glycosyltransferases that were not highly homologous to the 35000HP\n            <jats:italic>lbgAB<\/jats:italic>\n            genes. In this study, we demonstrate that the product of one of these genes encodes a galactosyltransferase. In addition, dot blot hybridization determined that 3 of 35 strains tested had the atypical transferases present, as did 4 strains characterized as class II strains by other criterion. These data indicate that the\n            <jats:italic>lbgAB<\/jats:italic>\n            genes can serve as one indicator of the classification of\n            <jats:italic>H. ducreyi<\/jats:italic>\n            strains.\n          <\/jats:p>","DOI":"10.1128\/iai.01016-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"113-121","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Identification of Genes Involved in the Expression of Atypical Lipooligosaccharide Structures from a Second Class of\n            <i>Haemophilus ducreyi<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Deborah M. 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