{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:40:46Z","timestamp":1782747646362,"version":"3.54.5"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Brucellosis is an important zoonotic disease of nearly worldwide distribution. Despite the availability of live vaccine strains for bovine (S19, RB51) and small ruminants (Rev-1), these vaccines have several drawbacks, including residual virulence for animals and humans. Safe and efficacious immunization systems are therefore needed to overcome these disadvantages. A\n            <jats:italic>vjbR<\/jats:italic>\n            knockout was generated in the S19 vaccine and investigated for its potential use as an improved vaccine candidate. Vaccination with a sustained-release vehicle to enhance vaccination efficacy was evaluated utilizing the live S19 \u0394\n            <jats:italic>vjbR<\/jats:italic>\n            ::Kan in encapsulated alginate microspheres containing a nonimmunogenic eggshell precursor protein of the parasite\n            <jats:italic>Fasciola hepatica<\/jats:italic>\n            (vitelline protein B). BALB\/c mice were immunized intraperitoneally with either encapsulated or nonencapsulated S19 \u0394\n            <jats:italic>vjbR<\/jats:italic>\n            ::Kan at a dose of 1 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            CFU per animal to evaluate immunogenicity, safety, and protective efficacy. Humoral responses postvaccination indicate that the vaccine candidate was able to elicit an anti-\n            <jats:italic>Brucella<\/jats:italic>\n            -specific immunoglobulin G response even when the vaccine was administered in an encapsulated format. The safety was revealed by the absence of splenomegaly in mice that were inoculated with the mutant. Finally, a single dose with the encapsulated mutant conferred higher levels of protection compared to the nonencapsulated vaccine. These results suggest that S19 \u0394\n            <jats:italic>vjbR<\/jats:italic>\n            ::Kan is safer than S19, induces protection in mice, and should be considered as a vaccine candidate when administered in a sustained-release manner.\n          <\/jats:p>","DOI":"10.1128\/iai.01017-08","type":"journal-article","created":{"date-parts":[[2008,12,2]],"date-time":"2008-12-02T01:59:42Z","timestamp":1228183182000},"page":"877-884","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["The Brucella abortus S19 \u0394\n            <i>vjbR<\/i>\n            Live Vaccine Candidate Is Safer than S19 and Confers Protection against Wild-Type Challenge in BALB\/c Mice When Delivered in a Sustained-Release Vehicle"],"prefix":"10.1128","volume":"77","author":[{"given":"A. M.","family":"Arenas-Gamboa","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. A.","family":"Ficht","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Kahl-McDonagh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Rice-Ficht","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&amp;M University, College Station, Texas"},{"name":"Department of Cellular and Molecular Medicine, College of Medicine, Texas A&amp;M University Health Science Center, College Station, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/10837459609031419"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1008-1016.1998"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.84.5.104"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00767-07"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00165-X"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/313812"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00233-X"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00543.x"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/02652040500435253"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1208\/s12249-008-9149-2"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.07.004"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/10611860410001670017"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4102-4107.2000"},{"key":"e_1_3_1_15_2","first-page":"211","volume":"87","year":"2003","unstructured":"Jones, D. 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