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In this regard,<jats:italic>B. burgdorferi<\/jats:italic>must adapt to various environmental signals, pHs, temperatures, and O<jats:sub>2<\/jats:sub>and CO<jats:sub>2<\/jats:sub>levels to establish infectious foci. We hypothesize that the BosR protein functions as a global regulator that is required for both borrelial oxidative homeostasis and pathogenesis. To assess the role of BosR in<jats:italic>B. burgdorferi<\/jats:italic>, we constructed an IPTG (isopropyl-\u03b2-<jats:sc>d<\/jats:sc>-thiogalactopyranoside)-regulated<jats:italic>bosR<\/jats:italic>strain. The selective decrease of<jats:italic>bosR<\/jats:italic>resulted in a change in growth when cells were cultured either anaerobically or microaerobically; however, a distinct growth defect was observed for anaerobically grown<jats:italic>B. burgdorferi<\/jats:italic>relative to the growth attenuation observed for microaerobically grown<jats:italic>B. burgdorferi<\/jats:italic>.<jats:italic>B. burgdorferi<\/jats:italic>cells in which BosR levels were reduced were more sensitive to hydrogen peroxide and produced lower levels of NapA (Dps) and SodA, proteins involved in the oxidative stress response. In addition, the levels of OspC and DbpA were also induced coincident with increased BosR levels, suggesting that BosR interfaces with the RpoS regulatory cascade, which is known to modulate virulence gene expression in<jats:italic>B. burgdorferi<\/jats:italic>. Taken together, these results indicate that BosR is involved in the resistance of<jats:italic>B. burgdorferi<\/jats:italic>to oxidative stressors and affects the expression of genes, either directly or indirectly, whose products are important in borrelial pathogenesis.<\/jats:p>","DOI":"10.1128\/iai.01018-09","type":"journal-article","created":{"date-parts":[[2009,10,27]],"date-time":"2009-10-27T01:29:57Z","timestamp":1256606997000},"page":"265-274","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Characterization of a Conditional<i>bosR<\/i>Mutant in<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"78","author":[{"given":"Jenny A.","family":"Hyde","sequence":"first","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana K.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of 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