{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:43:34Z","timestamp":1791020614786,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Animal models of infective endocarditis (IE) induced by high-grade bacteremia revealed the pathogenic roles of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n                    surface adhesins and platelet aggregation in the infection process. In humans, however,\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    IE possibly occurs through repeated bouts of low-grade bacteremia from a colonized site or intravenous device. Here we used a rat model of IE induced by continuous low-grade bacteremia to explore further the contributions of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    virulence factors to the initiation of IE. Rats with aortic vegetations were inoculated by continuous intravenous infusion (0.0017 ml\/min over 10 h) with 10\n                    <jats:sup>6<\/jats:sup>\n                    CFU of\n                    <jats:italic>Lactococcus lactis<\/jats:italic>\n                    pIL253 or a recombinant\n                    <jats:italic>L. lactis<\/jats:italic>\n                    strain expressing an individual\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    surface protein (ClfA, FnbpA, BCD, or SdrE) conferring a particular adhesive or platelet aggregation property. Vegetation infection was assessed 24 h later. Plasma was collected at 0, 2, and 6 h postinoculation to quantify the expression of tumor necrosis factor (TNF), interleukin 1\u03b1 (IL-1\u03b1), IL-1\u03b2, IL-6, and IL-10. The percentage of vegetation infection relative to that with strain pIL253 (11%) increased when binding to fibrinogen was conferred on\n                    <jats:italic>L. lactis<\/jats:italic>\n                    (ClfA strain) (52%;\n                    <jats:italic>P<\/jats:italic>\n                    = 0.007) and increased further with adhesion to fibronectin (FnbpA strain) (75%;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). Expression of fibronectin binding alone was not sufficient to induce IE (BCD strain) (10% of infection). Platelet aggregation increased the risk of vegetation infection (SdrE strain) (30%). Conferring adhesion to fibrinogen and fibronectin favored IL-1\u03b2 and IL-6 production. Our results, with a model of IE induced by low-grade bacteremia, resembling human disease, extend the essential role of fibrinogen binding in the initiation of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    IE. Triggering of platelet aggregation or an inflammatory response may contribute to or promote the development of IE.\n                  <\/jats:p>","DOI":"10.1128\/iai.01030-12","type":"journal-article","created":{"date-parts":[[2012,12,17]],"date-time":"2012-12-17T23:19:30Z","timestamp":1355786370000},"page":"697-703","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Use of a Human-Like Low-Grade Bacteremia Model of Experimental Endocarditis To Study the Role of Staphylococcus aureus Adhesins and Platelet Aggregation in Early Endocarditis"],"prefix":"10.1128","volume":"81","author":[{"given":"Tiago Rafael","family":"Veloso","sequence":"first","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz","family":"Chaouch","sequence":"additional","affiliation":[{"name":"Statistical Unit, Institute for Social and Preventive Medicine, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Roger","sequence":"additional","affiliation":[{"name":"Infectious Diseases Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlyse","family":"Giddey","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Vouillamoz","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Majcherczyk","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yok-Ai","family":"Que","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Rousson","sequence":"additional","affiliation":[{"name":"Statistical Unit, Institute for Social and Preventive Medicine, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Moreillon","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Entenza","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2011.43"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(01)00009-5"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01400-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1425"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04922.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04731.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01993-06"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3120-3127.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.1.65"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-12-3387"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02935.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.8.2306-2312.1989"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.12.4738-4743.1995"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00405-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050125"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00157.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/427663"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1160\/TH05-04-0235","article-title":"Mechanism and consequences of invasion of endothelial cells by Staphylococcus aureus","volume":"94","author":"Sinha B","year":"2005","unstructured":"SinhaBHerrmannM. 2005. 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