{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:11:52Z","timestamp":1791047512272,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter jejuni<\/jats:named-content>\n            is a natural commensal of the avian intestinal tract. However, the bacterium is also the leading cause of acute bacterial diarrhea worldwide and is implicated in development of Guillain-Barr\u00e9 syndrome. Like many bacterial pathogens,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            assembles complex surface structures that interface with the surrounding environment and are involved in pathogenesis. Recent work in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            identified a gene encoding a novel phosphoethanolamine (pEtN) transferase, EptC (Cj0256), that plays a promiscuous role in modifying the flagellar rod protein, FlgG; the lipid A domain of lipooligosaccharide (LOS); and several\n            <jats:italic>N<\/jats:italic>\n            -linked glycans. In this work, we report that EptC catalyzes the addition of pEtN to the first heptose sugar of the inner core oligosaccharide of LOS, a fourth enzymatic target. We also examine the role pEtN modification plays in circumventing detection and\/or killing by host defenses. Specifically, we show that modification of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            lipid A with pEtN results in increased recognition by the human Toll-like receptor 4\u2013myeloid differentiation factor 2 (hTLR4-MD2) complex, along with providing resistance to relevant mammalian and avian antimicrobial peptides (i.e., defensins). We also confirm the inability of aberrant forms of LOS to activate Toll-like receptor 2 (TLR2). Most exciting, we demonstrate that strains lacking\n            <jats:italic>eptC<\/jats:italic>\n            show decreased commensal colonization of chick ceca and reduced colonization of BALB\/cByJ mice compared to wild-type strains. Our results indicate that modification of surface structures with pEtN by EptC is key to its ability to promote commensalism in an avian host and to survive in the mammalian gastrointestinal environment.\n          <\/jats:p>","DOI":"10.1128\/iai.01046-12","type":"journal-article","created":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T05:57:04Z","timestamp":1353995824000},"page":"430-440","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["EptC of Campylobacter jejuni Mediates Phenotypes Involved in Host Interactions and Virulence"],"prefix":"10.1128","volume":"81","author":[{"given":"Thomas W.","family":"Cullen","sequence":"first","affiliation":[{"name":"Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Hendrixson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K.","family":"Giles","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences, University of Tennessee at Chattanooga, Chattanooga, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda I.","family":"Hobb","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Georgia Health Sciences University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart A.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Georgia Health Sciences University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer S.","family":"Brodbelt","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Stephen","family":"Trent","sequence":"additional","affiliation":[{"name":"Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas, USA"},{"name":"Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA, University of Texas at Austin, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/381583"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1202-1208.2002"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301273200"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00079-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00378-09"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05100.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.010307.145803"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519060120040201"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913451107"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.321737"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.380212"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5540-2"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00897-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.102061"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni777"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1201193109"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002454"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01222-09"},{"key":"e_1_3_3_20_2","first-page":"83","volume-title":"Methods in carbohydrate chemistry","author":"Westphal O","year":"1965","unstructured":"WestphalOJannK. 1965. Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure, p 83\u201391. In WhistlerRL (ed), Methods in carbohydrate chemistry, vol 5. Academic Press, Inc., New York, NY."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.618"},{"key":"e_1_3_3_22_2","volume-title":"Department of Microbiology and Immunology","author":"Hancock REW","year":"1999","unstructured":"HancockREW. 19 September 1999, posting date. Hancock Laboratory methods: modified MIC method for cationic antimicrobial peptides. Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada. http:\/\/www.cmdr.ubc.ca\/bobh\/methods.htm."},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.03988.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)80006-Y"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06161.x"},{"key":"e_1_3_3_26_2","first-page":"21202","article-title":"A phosphoethanolamine transferase specific for the outer 3-deoxy-d-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide","volume":"280","author":"Reynolds CM","year":"2005","unstructured":"ReynoldsCMKalbSRCotterRJRaetzCR. 2005. A phosphoethanolamine transferase specific for the outer 3-deoxy-d-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J. Biol. Chem. 280:21202\u201321211.","journal-title":"Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J. Biol. Chem."},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3391-3399.2005"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02754.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6970-6982.2004"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.2174\/138161209788682325"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00568-06"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406480200"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07304.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00667-08"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00146-06"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.441-444.1991"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1209775"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/86354"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-6-1171"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.938-943.1998"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3731-3735.1995"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01297-10"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402397101"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/35001088"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4124-4133.2004"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1200729"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000953"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07830"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400062653"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06044.x"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01046-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01046-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:14:01Z","timestamp":1646424841000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01046-12"}},"subtitle":[],"editor":[{"given":"S. R.","family":"Blanke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1128\/IAI.01046-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.01046-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"2012-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}