{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T04:51:04Z","timestamp":1679028664035},"reference-count":59,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            is a facultative intracellular fungal pathogen that has a polysaccharide capsule and causes life-threatening meningoencephalitis. Its capsule, as well as its ability to survive in the acidic environment of the phagolysosome, contributes to the pathogen's resilience in the host environment. Previously, we reported that downregulation of allergen 1 (\n            <jats:italic>ALL1<\/jats:italic>\n            ) results in the secretion of a shorter, more viscous exopolysaccharide with less branching and structural complexity, as well as altered iron homeostasis. Now, we report on a homologous coregulated gene, allergen 2 (\n            <jats:italic>ALL2<\/jats:italic>\n            ).\n            <jats:italic>ALL2<\/jats:italic>\n            's function was characterized by generating null mutants in\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            . In contrast to\n            <jats:italic>ALL1<\/jats:italic>\n            , loss of\n            <jats:italic>ALL2<\/jats:italic>\n            attenuated virulence in the pulmonary infection model. The\n            <jats:italic>all2<\/jats:italic>\n            \u0394 mutant shed a less viscous exopolysaccharide and exhibited higher sensitivity to hydrogen peroxide than the wild type, and as a result, the\n            <jats:italic>all2<\/jats:italic>\n            \u0394 mutant was more resistant to macrophage-mediated killing. Transcriptome analysis further supported the distinct function of these two genes. Unlike\n            <jats:italic>ALL1<\/jats:italic>\n            's involvement in iron homeostasis, we now present data on\n            <jats:italic>ALL2<\/jats:italic>\n            's unique function in maintaining intracellular pH in low-pH conditions. Thus, our data highlight that\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            , a human-pathogenic basidiomycete, has evolved a unique set of virulence-associated genes that contributes to its resilience in the human niche.\n          <\/jats:p>","DOI":"10.1128\/iai.01046-15","type":"journal-article","created":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T02:59:20Z","timestamp":1448333960000},"page":"439-451","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["<i>ALL2<\/i>\n            , a Homologue of\n            <i>ALL1<\/i>\n            , Has a Distinct Role in Regulating pH Homeostasis in the Pathogen Cryptococcus neoformans"],"prefix":"10.1128","volume":"84","author":[{"given":"Neena","family":"Jain","sequence":"first","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tejas","family":"Bouklas","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjali","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Albert Einstein College of Medicine, Bronx, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avanish K.","family":"Varshney","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika P.","family":"Orner","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina C.","family":"Fries","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Stony Brook University, Stony Brook, New York, USA"},{"name":"Department of Microbiology and Molecular Genetics, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328322ffac"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/518607"},{"key":"e_1_3_2_4_2","first-page":"19","article-title":"Virulence factors of Cryptococcus neoformans","volume":"7","author":"Hamilton AJ","year":"1996","unstructured":"Hamilton AJ, Goodley J. 1996. 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