{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:21:46Z","timestamp":1790695306743,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1062383"],"award-info":[{"award-number":["1062383"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1063646"],"award-info":[{"award-number":["1063646"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n            , the causative agent of Q fever, establishes a unique lysosome-derived intracellular niche termed the\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            -containing vacuole (CCV). The Dot\/Icm-type IVB secretion system is essential for the biogenesis of the CCV and the intracellular replication of\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            . Effector proteins, translocated into the host cell through this apparatus, act to modulate host trafficking and signaling processes to facilitate CCV development. Here we investigated the role of CBU0077, a conserved\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            effector that had previously been observed to localize to lysosomal membranes. CBU0077 was dispensable for the intracellular replication of\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            in HeLa and THP-1 cells and did not appear to participate in CCV biogenesis. Intriguingly, native and epitope-tagged CBU0077 produced by\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            displayed specific punctate localization at host cell mitochondria. As such, we designated CBU0077 MceA (\n            <jats:underline>m<\/jats:underline>\n            itochondrial\n            <jats:underline>\n              <jats:italic>C<\/jats:italic>\n            <\/jats:underline>\n            <jats:named-content content-type=\"genus-species\">oxiella<\/jats:named-content>\n            <jats:underline>e<\/jats:underline>\n            ffector protein\n            <jats:underline>A<\/jats:underline>\n            ). Analysis of ectopically expressed MceA truncations revealed that the capacity to traffic to mitochondria is encoded within the first 84 amino acids of this protein. MceA is farnesylated by the host cell; however, this does not impact mitochondrial localization. Examination of mitochondria isolated from infected cells revealed that MceA is specifically integrated into the mitochondrial outer membrane and forms a complex of approximately 120 kDa. Engineering\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            to express either MceA tagged with 3\u00d7FLAG or MceA tagged with 2\u00d7hemagglutinin allowed us to perform immunoprecipitation experiments that showed that MceA forms a homo-oligomeric species at the mitochondrial outer membrane during infection. This research reveals that mitochondria are a bona fide target of\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            effectors and MceA is a complex-forming effector at the mitochondrial outer membrane during\n            <jats:named-content content-type=\"genus-species\">Coxiella<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.01046-16","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T01:40:18Z","timestamp":1488246018000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["A Farnesylated Coxiella burnetii Effector Forms a Multimeric Complex at the Mitochondrial Outer Membrane during Infection"],"prefix":"10.1128","volume":"85","author":[{"given":"Laura F.","family":"Fielden","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer H.","family":"Moffatt","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen Ai","family":"Khoo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig R.","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-3222","authenticated-orcid":false,"given":"Diana","family":"Stojanovski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 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