{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:16Z","timestamp":1789996516017,"version":"4.0.1"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteoarticular brucellosis is the most common localization of human active disease. Osteocytes are the most abundant cells of bone. They secrete factors that regulate the differentiation of both osteoblasts and osteoclasts during bone remodeling. The aim of this study is to determine if\n                    <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n                    infection modifies osteocyte function. Our results indicate that\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    infection induced matrix metalloproteinase 2 (MMP-2), receptor activator for NF-\u03baB ligand (RANKL), proinflammatory cytokines, and keratinocyte chemoattractant (KC) secretion by osteocytes. In addition, supernatants from\n                    <jats:named-content content-type=\"genus-species\">B. abortus-<\/jats:named-content>\n                    infected osteocytes induced bone marrow-derived monocytes (BMM) to undergo osteoclastogenesis. Using neutralizing antibodies against tumor necrosis factor alpha (TNF-\u03b1) or osteoprotegerin (OPG), RANKL's decoy receptor, we determined that TNF-\u03b1 and RANKL are involved in osteoclastogenesis induced by supernatants from\n                    <jats:named-content content-type=\"genus-species\">B. abortus-<\/jats:named-content>\n                    infected osteocytes. Connexin 43 (Cx43) and the integrins E11\/gp38, integrin-\u03b1, integrin-\u03b2, and CD44 are involved in cell-cell interactions necessary for osteocyte survival.\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    infection inhibited the expression of Cx43 but did not modify the expression of integrins. Yet the expression of both Cx43 and integrins was inhibited by supernatants from\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    -infected macrophages.\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    infection was not capable of inducing osteocyte apoptosis. However, supernatants from\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    -infected macrophages induced osteocyte apoptosis in a dose-dependent manner. Taken together, our results indicate that\n                    <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n                    infection could alter osteocyte function, contributing to bone damage.\n                  <\/jats:p>","DOI":"10.1128\/iai.01049-15","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T14:40:10Z","timestamp":1444747210000},"page":"11-20","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Brucella abortus Invasion of Osteocytes Modulates Connexin 43 and Integrin Expression and Induces Osteoclastogenesis via Receptor Activator of NF-\u03baB Ligand and Tumor Necrosis Factor Alpha Secretion"],"prefix":"10.1128","volume":"84","author":[{"given":"Ayel\u00e9n Ivana","family":"Pesce Viglietti","sequence":"first","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Constanza","family":"Arriola Benitez","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Virginia","family":"Gentilini","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lis Noelia","family":"Vel\u00e1squez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental, CONICET-Academia Nacional de Medicina, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"Fossati","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos, CONICET-Universidad Nacional de La Plata, Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, La Plata, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo Hern\u00e1n","family":"Giambartolomei","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Victoria","family":"Delpino","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800630408"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197201000-00013"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.57187\/smw.2002.09900","article-title":"Musculoskeletal involvement of brucellosis in different age groups: a study of 195 cases","volume":"132","author":"Geyik MF","year":"2002","unstructured":"Geyik MF, Gur A, Nas K, Cevik R, Sarac J, Dikici B, Ayaz C. 2002. 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