{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:37:19Z","timestamp":1791405439066,"version":"4.3.3"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Braun (murein) lipoprotein (Lpp) and lipopolysaccharide (LPS) are major components of the outer membranes of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>family members that are capable of triggering inflammatory immune responses by activating Toll-like receptors 2 and 4, respectively. Expanding on earlier studies that demonstrated a role played by Lpp in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia pestis<\/jats:named-content>virulence in mouse models of bubonic and pneumonic plague, we characterized an<jats:italic>msbB<\/jats:italic>in-frame deletion mutant incapable of producing an acyltransferase that is responsible for the addition of lauric acid to the lipid A moiety of LPS, as well as a \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant of the highly virulent<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pestis<\/jats:named-content>CO92 strain. Although the \u0394<jats:italic>msbB<\/jats:italic>single mutant was minimally attenuated, the \u0394<jats:italic>lpp<\/jats:italic>single mutant and the \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant were significantly more attenuated than the isogenic wild-type (WT) bacterium in bubonic and pneumonic animal models (mouse and rat) of plague. These data correlated with greatly reduced survivability of the aforementioned mutants in murine macrophages. Furthermore, the \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant was grossly compromised in its ability to disseminate to distal organs in mice and in evoking cytokines\/chemokines in infected animal tissues. Importantly, mice that survived challenge with the \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant, but not the \u0394<jats:italic>lpp<\/jats:italic>or \u0394<jats:italic>msbB<\/jats:italic>single mutant, in a pneumonic plague model were significantly protected against a subsequent lethal WT CO92 rechallenge. These data were substantiated by the fact that the \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant maintained an immunogenicity comparable to that of the WT strain and induced long-lasting T-cell responses against heat-killed WT CO92 antigens. Taken together, the data indicate that deletion of the<jats:italic>msbB<\/jats:italic>gene augmented the attenuation of the \u0394<jats:italic>lpp<\/jats:italic>mutant by crippling the spread of the double mutant to the peripheral organs of animals and by inducing cytokine\/chemokine responses. Thus, the \u0394<jats:italic>lpp<\/jats:italic>\u0394<jats:italic>msbB<\/jats:italic>double mutant could provide a new live-attenuated background vaccine candidate strain, and this should be explored in the future.<\/jats:p>","DOI":"10.1128\/iai.01067-12","type":"journal-article","created":{"date-parts":[[2012,12,29]],"date-time":"2012-12-29T04:55:08Z","timestamp":1356756908000},"page":"815-828","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Deletion of the Braun Lipoprotein-Encoding Gene and Altering the Function of Lipopolysaccharide Attenuate the Plague Bacterium"],"prefix":"10.1128","volume":"81","author":[{"given":"Jian","family":"Sha","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Kirtley","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina J.","family":"van Lier","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaofei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana E.","family":"Erova","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Kozlova","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzi","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric C.","family":"Fitts","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Rosenzweig","sequence":"additional","affiliation":[{"name":"Department of Biology, Center for Bionanotechnology and Environmental Research (CBER), Texas Southern University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Chopra","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Galveston National Laboratory, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12320-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12270-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)05163-6"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2009.09.002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.09.129"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)91452-Z"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.43.070174.000513"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00763177"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1381-1388.2006"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.593-656.2003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118423"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.20.12095"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.16.10353"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46880-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05044-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01417-09"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048430f"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04059.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4092-4098.2002"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1386"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/35097083"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4611-4623.1998"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.16.4601-4611.2002"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.9734\/IJBCRR\/2011\/154"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.19.10.2517"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700686"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.9.5231"},{"key":"e_1_3_3_29_2","doi-asserted-by":"crossref","first-page":"4868","DOI":"10.4049\/jimmunol.159.10.4868","article-title":"Bacterial lipoprotein and lipopolysaccharide act synergistically to induce lethal shock and proinflammatory cytokine production","volume":"159","author":"Zhang H","year":"1997","unstructured":"ZhangHPetersonJWNieselDWKlimpelGR. 1997. Bacterial lipoprotein and lipopolysaccharide act synergistically to induce lethal shock and proinflammatory cytokine production. J. Immunol. 159:4868\u20134878.","journal-title":"J. Immunol."},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5428.736"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.02.009"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.11.1777"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135414"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2085"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8541"},{"key":"e_1_3_3_36_2","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.4049\/jimmunol.162.7.3749","article-title":"Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product","volume":"162","author":"Hoshino K","year":"1999","unstructured":"HoshinoKTakeuchiOKawaiTSanjoHOgawaTTakedaYTakedaKAkiraS. 1999. Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J. Immunol. 162:3749\u20133752.","journal-title":"J. Immunol."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3380-6"},{"key":"e_1_3_3_38_2","volume-title":"Plague immunology","author":"Naumov AV","year":"1992","unstructured":"NaumovAVLedvanovMYDrozdovIG. 1992. Plague immunology. Russian Research Anti-Plague Institute, Saratov, Russia."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00163-3"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.020"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00273-09"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.01.099"},{"key":"e_1_3_3_43_2","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.4049\/jimmunol.142.9.3229","article-title":"IL-1 induction-capacity of defined lipopolysaccharide partial structures","volume":"142","author":"Loppnow H","year":"1989","unstructured":"LoppnowHBradeHDurrbaumIDinarelloCAKusumotoSRietschelETFladHD. 1989. IL-1 induction-capacity of defined lipopolysaccharide partial structures. J. Immunol. 142:3229\u20133238.","journal-title":"J. Immunol."},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55023-7"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01529-07"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00619-7"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00064-11"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8433-8436.2005"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1081-1096.2005"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.3987-4003.2004"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01125-07"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00352-07"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1016-1024.2006"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.11.009"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00533-09"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2007.09.005"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.20.1.142"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.08.055"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.647-655.2003"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni777"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6132-6140.2003"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/342168"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01517-07"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45932-0"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5554-5561.2001"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01067-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01067-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T07:30:22Z","timestamp":1687937422000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01067-12"}},"subtitle":[],"editor":[{"given":"B. A.","family":"McCormick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":64,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1128\/IAI.01067-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.01067-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]},"assertion":[{"value":"2012-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}