{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:12:54Z","timestamp":1761293574768,"version":"3.41.2"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31470242","31170122"],"award-info":[{"award-number":["31470242","31170122"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2013CB910804"],"award-info":[{"award-number":["2013CB910804"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pathogenic yersiniae harbor a type III secretion system (T3SS) that injects\n            <jats:named-content content-type=\"genus-species\">Yersinia<\/jats:named-content>\n            outer protein (Yop) into host cells. YopK has been shown to control Yop translocation and prevent inflammasome recognition of the T3SS by the innate immune system. Here, we demonstrate that YopK inhibits bacterial adherence to host cells by binding to the extracellular matrix adaptor protein matrilin-2 (MATN2). YopK binds to MATN2, and deleting amino acids 91 to 124 disrupts binding of YopK to MATN2. A\n            <jats:italic>yopK<\/jats:italic>\n            null mutant exhibits a hyperadhesive phenotype, which could be responsible for the established Yop hypertranslocation phenotype of\n            <jats:italic>yopK<\/jats:italic>\n            mutants. Expression of YopK, but not YopK\n            <jats:sub>\u039491\u2013124<\/jats:sub>\n            , in a\n            <jats:italic>yopK<\/jats:italic>\n            mutant restored the wild-type phenotypes of adhesion and Yop translocation, suggesting that binding to MATN2 might be essential for YopK to inhibit bacterial adhesion and negatively regulate Yop translocation. A green fluorescent protein (GFP)-YopK fusion specifically binds to the endogenous MATN2 on the surface of HeLa cells, whereas GFP-YopK\n            <jats:sub>\u039491\u2013124<\/jats:sub>\n            cannot. Addition of purified YopK protein during infection decreased adhesion of\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            to HeLa cells, while YopK\n            <jats:sub>\u039491\u2013124<\/jats:sub>\n            protein showed no effect. Taking these results together, we propose a model that the T3SS-secreted YopK hinders bacterial adhesion to HeLa cells by binding to MATN2, which is ubiquitously exposed on eukaryotic cells.\n          <\/jats:p>","DOI":"10.1128\/iai.01069-16","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:30:24Z","timestamp":1495499424000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Yersinia pestis YopK Inhibits Bacterial Adhesion to Host Cells by Binding to the Extracellular Matrix Adaptor Protein Matrilin-2"],"prefix":"10.1128","volume":"85","author":[{"given":"Yafang","family":"Tan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 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