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To assess whether intracellular signals might influence the interaction between ANTXR1 and PA, we compared two splice variants of ANTXR1 that differ only in their cytoplasmic domains. We found that cells expressing ANTXR1 splice variant 1 (ANTXR1-sv1) bound markedly less PA than did cells expressing a similar level of the shorter splice variant ANTXR1-sv2. ANTXR1-sv1 but not ANTXR1-sv2 associated with the actin cytoskeleton, although disruption of the cytoskeleton did not affect binding of ANTXR-sv1 to PA. Introduction of a cytoplasmic domain missense mutation found in the related receptor ANTXR2 in a patient with juvenile hyaline fibromatosis impaired actin association and increased binding of PA to ANTXR1-sv1. These results suggest that ANTXR1 has two affinity states that may be modulated by cytoplasmic signals.<\/jats:p>","DOI":"10.1128\/iai.01073-08","type":"journal-article","created":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T00:44:34Z","timestamp":1224549874000},"page":"52-59","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["The Cytoplasmic Domain of Anthrax Toxin Receptor 1 Affects Binding of the Protective Antigen"],"prefix":"10.1128","volume":"77","author":[{"given":"Mandy Y.","family":"Go","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith M. 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