{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:46:16Z","timestamp":1784119576101,"version":"3.55.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Bordetella c<\/jats:italic>olonization<jats:italic>f<\/jats:italic>actor<jats:italic>A<\/jats:italic>(BcfA) is an outer membrane immunogenic protein, which is critical for efficient colonization of the murine respiratory tract. These properties of BcfA prompted us to examine its utility in inducing a protective immune response against<jats:italic>Bordetella bronchiseptica<\/jats:italic>in a mouse model of intranasal infection. Mice vaccinated with BcfA demonstrated reduced pathology in the lungs and harbored lower bacterial burdens in the respiratory tract. Immunization with BcfA led to the generation of BcfA-specific antibodies in both the sera and lungs, and passive immunization led to the reduction of<jats:italic>B. bronchiseptica<\/jats:italic>in the tracheas and lungs. These results suggest that protection after immunization with BcfA is mediated in part by antibodies against BcfA. To further investigate the mechanism of BcfA-induced immune clearance, we examined the role of neutrophils and macrophages. Our results demonstrate that neutrophils are critical for anti-BcfA antibody-mediated clearance and that opsonization with anti-BcfA serum enhances phagocytosis of<jats:italic>B. bronchiseptica<\/jats:italic>by murine macrophages. We show that immunization with BcfA results in the production of gamma interferon and subclasses of immunoglobulin G antibodies that are consistent with the induction of a Th1-type immune response. In combination, our findings suggest that the mechanism of BcfA-mediated immunity involves humoral and cellular responses. Expression of BcfA is conserved among multiple clinical isolates of<jats:italic>B. bronchiseptica<\/jats:italic>. Our results demonstrate the striking protective efficacy of BcfA-mediated immunization, thereby highlighting its utility as a potential vaccine candidate. These results also provide a model for the development of cell-free vaccines against<jats:italic>B. bronchiseptica<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/iai.01076-08","type":"journal-article","created":{"date-parts":[[2008,12,9]],"date-time":"2008-12-09T01:25:40Z","timestamp":1228785940000},"page":"885-895","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Active and Passive Immunizations with<i>Bordetella<\/i>Colonization Factor A Protect Mice against Respiratory Challenge with<i>Bordetella bronchiseptica<\/i>"],"prefix":"10.1128","volume":"77","author":[{"given":"Neelima","family":"Sukumar","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheraton F.","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt S.","family":"Conover","sequence":"additional","affiliation":[{"name":"Program in Molecular Genetics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy D.","family":"Kock","sequence":"additional","affiliation":[{"name":"Comparative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, North Carolina 27157"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purnima","family":"Dubey","sequence":"additional","affiliation":[{"name":"Department of Pathology\/"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendar","family":"Deora","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Program in Molecular Genetics"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.497560.x"},{"key":"e_1_3_2_3_2","first-page":"1130","volume":"42","year":"1981","unstructured":"Bey, R. F., F. J. Shade, R. A. Goodnow, and R. C. Johnson. 1981. Intranasal vaccination of dogs with liver avirulent Bordetella bronchiseptica: correlation of serum agglutination titer and the formation of secretory IgA with protection against experimentally induced infectious tracheobronchitis. Am. J. Vet. Res.42:1130-1132.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.05.022"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00226-08"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006058"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2006.12.004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01163-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)31230-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3821741.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240726"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.4049\/jimmunol.152.4.1836","volume":"152","year":"1994","unstructured":"Czuprynski, C. J., J. F. Brown, N. Maroushek, R. D. Wagner, and H. Steinberg. 1994. Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection. J. Immunol.152:1836-1846.","journal-title":"J. Immunol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.1.70-75.1990"},{"key":"e_1_3_2_14_2","first-page":"260","volume":"56","year":"1992","unstructured":"Dugal, F., M. Belanger, and M. Jacques. 1992. Enhanced adherence of Pasteurella multocida to porcine tracheal rings preinfected with Bordetella bronchiseptica. Can. J. Vet. Res.56:260-264.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/514761"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.54.1105"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00836-07"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.44.4.722-738.1980"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.01.001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1996.d01-30.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.8.2002-2006.1995"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6109-6118.1999"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199404000-00011"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.39-49.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04958.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7212-7219.2004"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00510-06"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1719-1724.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.11.7504"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4789-4791.2000"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01648-06"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00162-9"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(02)02288-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.282.6276.1595"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.594-602.1998"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00801.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1043-1049.2006"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-2-255"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1086\/528858"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.8.3190-3198.1993"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1568-1579.2004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.6.1914-1918.1990"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01927-05"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00785-07"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2591-2597.2003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.01968.x"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-63-2-211"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00118-4"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00068-3"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00009-07"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4673-4680.2000"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.3.243"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01076-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01076-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T19:09:01Z","timestamp":1684782541000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01076-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1128\/IAI.01076-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.01076-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2]]},"assertion":[{"value":"2008-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}