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Utilizing an engineered strain of the intracellular pathogen<jats:italic>Listeria monocytogenes<\/jats:italic>that infects cells but fails to escape from the phagosome, we demonstrate the induction of long-lived memory T cells that are capable of secondary expansion and effector function but are incapable of providing protective immunity. We demonstrate that microbial invasion of the cytosol is required for dendritic cell activation and integration of CD40 signaling, ultimately determining the ability of the elicited CD8<jats:sup>+<\/jats:sup>-T-cell pool to protect against lethal wild-type<jats:italic>L. monocytogenes<\/jats:italic>challenge. These results reveal a crucial role for phagosomal escape, not for delivery of antigen to the class I major histocompatibility complex pathway but for establishing the appropriate cellular context during CD8<jats:sup>+<\/jats:sup>-T-cell priming.<\/jats:p>","DOI":"10.1128\/iai.01088-06","type":"journal-article","created":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T22:57:47Z","timestamp":1161385067000},"page":"6387-6397","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Cytosolic Entry Controls CD8<sup>+<\/sup>-T-Cell Potency during Bacterial Infection"],"prefix":"10.1128","volume":"74","author":[{"given":"Keith S.","family":"Bahjat","sequence":"first","affiliation":[{"name":"Cerus Corporation, Concord, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiqun","family":"Liu","sequence":"additional","affiliation":[{"name":"Cerus Corporation, Concord, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward E.","family":"Lemmens","sequence":"additional","affiliation":[{"name":"Cerus Corporation, Concord, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"Schoenberger","sequence":"additional","affiliation":[{"name":"Division of Cellular Immunology, La Jolla Institute for Allergy &amp; Immunology, San Diego, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Thomas W.","family":"Dubensky","sequence":"additional","affiliation":[{"name":"Cerus Corporation, Concord, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk G.","family":"Brockstedt","sequence":"additional","affiliation":[{"name":"Cerus Corporation, Concord, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/32183"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040976"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.4.1625-1632.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.1.196"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.1996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"3813","DOI":"10.4049\/jimmunol.139.11.3813","volume":"139","year":"1987","unstructured":"Berche, P., J. 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