{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:20Z","timestamp":1791343520416,"version":"4.3.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n The Ros-type regulator MucR is one of the few transcriptional regulators that have been linked to virulence in\n Brucella<\/jats:named-content>\n . Here, we show that a\n Brucella abortus<\/jats:named-content>\n in-frame\n mucR<\/jats:italic>\n deletion strain exhibits a pronounced growth defect during\n in vitro<\/jats:italic>\n cultivation and, more importantly, that the\n mucR<\/jats:italic>\n mutant is attenuated in cultured macrophages and in mice. The genetic basis for the attenuation of\n Brucella mucR<\/jats:italic>\n mutants has not been defined previously, but in the present study the genes regulated by MucR in\n B. abortus<\/jats:named-content>\n have been elucidated using microarray analysis and real-time reverse transcription-PCR (RT-PCR). In\n B. abortus<\/jats:named-content>\n 2308, MucR regulates a wide variety of genes whose products may function in establishing and maintaining cell envelope integrity, polysaccharide biosynthesis, iron homeostasis, genome plasticity, and transcriptional regulation. Particularly notable among the MucR-regulated genes identified is\n arsR6<\/jats:italic>\n (\n nolR<\/jats:italic>\n ), which encodes a transcriptional regulator previously linked to virulence in\n Brucella melitensis<\/jats:named-content>\n 16 M. Importantly, electrophoretic mobility shift assays (EMSAs) determined that a recombinant MucR protein binds directly to the promoter regions of several genes repressed by MucR (including\n arsR6<\/jats:italic>\n [\n nolR<\/jats:italic>\n ]), and in\n Brucella<\/jats:named-content>\n , as in other alphaproteobacteria, MucR binds to its own promoter to repress expression of the gene that encodes it. Overall, these studies have uncovered the diverse genetic regulon of MucR in\n Brucella<\/jats:named-content>\n , and in doing so this work has begun to define the MucR-controlled genetic circuitry whose misregulation contributes to the virulence defect of\n Brucella mucR<\/jats:italic>\n mutants.\n <\/jats:p>","DOI":"10.1128\/iai.01097-12","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T04:54:12Z","timestamp":1358225652000},"page":"1040-1051","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Diverse Genetic Regulon of the Virulence-Associated Transcriptional Regulator MucR in Brucella abortus 2308"],"prefix":"10.1128","volume":"81","author":[{"given":"Clayton C.","family":"Caswell","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed E. 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