{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T15:51:37Z","timestamp":1793634697643,"version":"4.5.1"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Enterotoxigenic\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    (ETEC) is a leading cause of death due to diarrheal illness among young children in developing countries, and there is currently no effective vaccine. Many elements of ETEC pathogenesis are still poorly defined. Here we demonstrate that YghJ, a secreted ETEC antigen identified in immunoproteomic studies using convalescent patient sera, is required for efficient access to small intestinal enterocytes and for the optimal delivery of heat-labile toxin (LT). Furthermore, YghJ is a highly conserved metalloprotease that influences intestinal colonization of ETEC by degrading the major mucins in the small intestine, MUC2 and MUC3. Genes encoding YghJ and its cognate type II secretion system (T2SS), which also secretes LT, are highly conserved in ETEC and exist in other enteric pathogens, including other diarrheagenic\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n                    bacteria, suggesting that this mucin-degrading enzyme may represent a shared virulence feature of these important pathogens.\n                  <\/jats:p>","DOI":"10.1128\/iai.01106-13","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:01:25Z","timestamp":1384819285000},"page":"509-521","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":133,"title":["Enterotoxigenic Escherichia coli Secretes a Highly Conserved Mucin-Degrading Metalloprotease To Effectively Engage Intestinal Epithelial Cells"],"prefix":"10.1128","volume":"82","author":[{"given":"Qingwei","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pardeep","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim J.","family":"Vickers","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaullah","family":"Sheikh","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Microbial Pathogenesis Program, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warren G.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Rasko","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeticia","family":"Sistrunk","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Fleckenstein","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA"},{"name":"Molecular Microbiology and Microbial Pathogenesis Program, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri, USA"},{"name":"Medicine Service, Veterans Affairs Medical Center, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.10.002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00459-07"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.3.465-483.2005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.37"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.06.084"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1786-1794.2004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2245-2258.2006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00264-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00919-12"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00736.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000902"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2538"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06394-11"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00932-10"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-7-12"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.2"},{"key":"e_1_3_3_19_2","volume-title":"Using antibodies: a laboratory manual","author":"Harlow E","year":"1999","unstructured":"HarlowELaneDHarlowE. 1999. Using antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.251546"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00702340"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2011.03.002"},{"key":"e_1_3_3_23_2","first-page":"3","article-title":"Isolation of large gel-forming mucins","volume":"125","author":"Davies JR","year":"2000","unstructured":"DaviesJRCarlstedtI. 2000. Isolation of large gel-forming mucins. Methods Mol. Biol. 125:3\u201313.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.044529-0"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.278135"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.869-875.2006"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001428"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092152899"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0915077107"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsn026"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00619-08"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)90299-4"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-010-0532-9"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.5.1641"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.11.2822-2829.1988"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.13.6977"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1106920"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00710-10"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)48015-3"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1996.0070"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030287"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1947-1952.2001"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90520-D"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.211078"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5587-5596.1999"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101006108"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02997.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2008.04.006"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01494-08"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200012)185:3<408::AID-JCP11>3.0.CO;2-2"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2371"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.1.139-145.1985"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.4.1219-1227.1994"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.40.1.62-69.1983"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00364-12"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.12"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.12.3.656-667.1975"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.8.3.322-328.1973"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.2.409-420.1984"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197107012850101"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01078-13"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01106-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01106-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T15:24:28Z","timestamp":1646407468000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01106-13"}},"subtitle":[],"editor":[{"given":"S. M.","family":"Payne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,2]]},"references-count":60,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1128\/IAI.01106-13"],"URL":"https:\/\/doi.org\/10.1128\/iai.01106-13","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2]]},"assertion":[{"value":"2013-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}