{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:01:28Z","timestamp":1793325688759,"version":"4.5.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Lipooligosaccharide (LOS) heptose (Hep) glycan substitutions influence gonococcal serum resistance. Several gonococcal strains bind the classical complement pathway inhibitor, C4b-binding protein (C4BP), via their porin (Por) molecule to escape complement-dependent killing by normal human serum (NHS). We show that the proximal glucose (Glc) on HepI is required for C4BP binding to Por1B-bearing gonococcal strains MS11 and 1291 but not to FA19 (Por1A). The presence of only the proximal Glc on HepI (<jats:italic>lgtE<\/jats:italic>mutant) permitted maximal C4BP binding to MS11 but not to 1291. Replacing 1291 lgtE Por with MS11 Por increased C4BP binding to levels that paralleled MS11 lgtE, suggesting that replacement of the Por1B molecule dictated the effects of HepI glycans on C4BP binding. The remainder of the strain background did not affect C4BP binding; replacing the Por of strain F62 with MS11 Por (F62 PorMS11) and truncating HepI mirrored the findings in the MS11 background. C4BP binding correlated with resistance to killing by NHS in most instances. F62 PorMS11 and its<jats:italic>lgtE<\/jats:italic>mutant were sensitive to NHS despite binding C4BP, secondary to kinetically overwhelming classical pathway activation and possibly increased alternative pathway activation (measured by factor Bb binding) by the F62 background. FA19 lgtF (HepI unsubstituted) resisted killing by only 10% NHS, not 50% NHS, despite binding levels of C4BP similar to those of FA19 and FA19 lgtE (both resistant to 50% serum), suggesting a role for the proximal Glc in serum resistance independently of C4BP binding. This study provides mechanistic insights into how HepI LOS substitutions affect the serum resistance of<jats:italic>N. gonorrhoeae<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/iai.01109-06","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T00:49:37Z","timestamp":1180140577000},"page":"4071-4081","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Heptose I Glycan Substitutions on<i>Neisseria gonorrhoeae<\/i>Lipooligosaccharide Influence C4b-Binding Protein Binding and Serum Resistance"],"prefix":"10.1128","volume":"75","author":[{"given":"Sanjay","family":"Ram","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutamas","family":"Ngampasutadol","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Cox","sequence":"additional","affiliation":[{"name":"National Research Council, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna M.","family":"Blom","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Section of Medical Protein Chemistry, Wallenberg Laboratory, University Hospital Malmo, Lund University, Malmo\u0308, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"St. Michael","sequence":"additional","affiliation":[{"name":"National Research Council, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Stupak","sequence":"additional","affiliation":[{"name":"National Research Council, Ottawa, Ontario K1A 0R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunita","family":"Gulati","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Rice","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.2.506"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10872"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.43.3.994-999.1984"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.6841"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0014-2956.2001.02459.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2090847"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.2.499-504.1988"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01439.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.4.1233"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.4.1044"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.150.2.267"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.1.17"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.12.6596"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.6.2181"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"298","DOI":"10.4049\/jimmunol.147.1.298","volume":"147","year":"1991","unstructured":"Griffiss, J. M., G. A. Jarvis, J. P. O'Brien, M. M. Eads, and H. Schneider. 1991. Lysis of Neisseria gonorrhoeae initiated by binding of normal human IgM to a hexosamine-containing lipooligosaccharide epitope(s) is augmented by strain-specific, properdin-binding-dependent alternative complement pathway activation. J. Immunol.147:298-305.","journal-title":"J. Immunol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7390-7397.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.6.1238"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.36.2.541-547.1982"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3230469"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02666.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01938.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5808-5815.2002"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54998-X"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.4049\/jimmunol.134.3.1920","volume":"134","year":"1985","unstructured":"Joiner, K. A., K. A. Warren, C. Hammer, and M. M. Frank. 1985. Bactericidal but not nonbactericidal C5b-9 is associated with distinctive outer membrane proteins in Neisseria gonorrhoeae. J. Immunol.134:1920-1925.","journal-title":"J. Immunol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5939-5947.1998"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.10.4439-4442.1992"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.4049\/jimmunol.161.7.3711","volume":"161","year":"1998","unstructured":"Liszewski, M. K., M. K. Leung, and J. P. Atkinson. 1998. Membrane cofactor protein: importance of N- and O-glycosylation for complement regulatory function. J. Immunol.161:3711-3718.","journal-title":"J. Immunol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.1.107"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1086\/314545"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)36013-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/145.2.206"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506471102"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1032"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90103-9"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.4.1498-1506.1995"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.11.6352"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308364200"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.3.281"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.4.671"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.5.743"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.989-997.1992"},{"key":"e_1_3_2_43_2","first-page":"145","volume":"27","year":"1961","unstructured":"Reyn, A. 1961. Sensitivity of Neisseria gonorrhoeae to antibiotics. Br. J. Vener. Dis.27:145-157.","journal-title":"Br. J. Vener. Dis."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.50.3.672-677.1985"},{"key":"e_1_3_2_45_2","first-page":"13","volume":"128","year":"1982","unstructured":"Schneider, H., J. M. Griffiss, G. D. Williams, and G. B. Pier. 1982. Immunological basis of serum resistance of Neisseria gonorrhoeae. J. Gen. Microbiol.128:13-22.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.149.4.870"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519020080010501"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00415519"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.5.1344"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2540626.x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2006.02323.x"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01109-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01109-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T01:13:04Z","timestamp":1683853984000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01109-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1128\/IAI.01109-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01109-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]},"assertion":[{"value":"2006-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}