{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:43:00Z","timestamp":1792852980713,"version":"4.5.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Transcriptional analysis of the<jats:italic>tpr<\/jats:italic>genes in<jats:italic>Treponema pallidum<\/jats:italic>subsp.<jats:italic>pallidum<\/jats:italic>(referred to here as simply<jats:italic>T. pallidum<\/jats:italic>) has been limited to date, and yet the expression of members of this gene family is likely relevant to the pathogenesis of syphilis. Recently, immunological studies and semiquantitative mRNA analysis led to the hypothesis of the modulation of<jats:italic>tpr<\/jats:italic>gene transcription during infection and suggested that various strains of<jats:italic>T. pallidum<\/jats:italic>might differentially express these genes. In this study we developed a real-time amplification assay to quantify the<jats:italic>tpr<\/jats:italic>mRNAs with respect to the 47-kDa lipoprotein message and to compare transcript levels among four different strains of<jats:italic>T. pallidum<\/jats:italic>. In addition, we analyzed the lymphocyte responsiveness pattern toward the Tpr antigens in late experimental syphilis to identify<jats:italic>tpr<\/jats:italic>genes that had been expressed during the course of infection. The T-cell response has been implicated in clearance of treponemes from early lesions, and some of the Tprs were identified as strong targets of the cellular immune response. We show that message for many of the<jats:italic>tpr<\/jats:italic>genes can be detected in treponemes harvested at the peak of early infection. Interestingly,<jats:italic>tprK<\/jats:italic>seems to be preferentially expressed in almost every strain, and it is uniformly the target of the strongest cellular immune response. These studies demonstrate the differential expression of certain<jats:italic>tpr<\/jats:italic>genes among strains of<jats:italic>T. pallidum<\/jats:italic>, and further studies are needed to explore the relationship between<jats:italic>tpr<\/jats:italic>gene expression and the clinical course of syphilis in infected individuals.<\/jats:p>","DOI":"10.1128\/iai.01124-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"104-112","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Quantitative Analysis of<i>tpr<\/i>Gene Expression in<i>Treponema pallidum<\/i>Isolates: Differences among Isolates and Correlation with T-Cell Responsiveness in Experimental Syphilis"],"prefix":"10.1128","volume":"75","author":[{"given":"Lorenzo","family":"Giacani","sequence":"first","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Molini","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charmie","family":"Godornes","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Barrett","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley","family":"Van Voorhis","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Centurion-Lara","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila A.","family":"Lukehart","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Pathobiology, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"4363","DOI":"10.4049\/jimmunol.141.12.4363","volume":"141","year":"1988","unstructured":"Baker-Zander, S. A., M. J. Fohn, and S. A. Lukehart. 1988. Development of cellular immunity to individual soluble antigens of Treponema pallidum during experimental syphilis. J. Immunol.141:4363-4369.","journal-title":"J. Immunol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1440-1444.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.4.647"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.824-831.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04086.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.8.2332-2335.2000"},{"key":"e_1_3_2_8_2","first-page":"102","volume":"69","year":"1993","unstructured":"Engelkens, H. J., F. J. ten Kate, J. Judanarso, V. D. Vuzevski, J. B. van Lier, J. C. Godschalk, J. J. van der Sluis, and E. Stolz. 1993. 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