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To date, the growth and nutritional requirements of fungi during infection, which are relevant for invasion of the host, are poorly understood. This is particularly true for invasive pulmonary aspergillosis, as so far, sources of (macro)elements that are exploited during infection have been identified to only a limited extent. Here, we have investigated sulfur (S) utilization by the human-pathogenic mold\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            during invasive growth. Our data reveal that inorganic S compounds or taurine is unlikely to serve as an S source during invasive pulmonary aspergillosis since a sulfate transporter mutant strain and a sulfite reductase mutant strain are fully virulent. In contrast, the S-containing amino acid cysteine is limiting for fungal growth, as proven by the reduced virulence of a cysteine auxotroph. Moreover, phenotypic characterization of this strain further revealed the robustness of the subordinate glutathione redox system. Interestingly, we demonstrate that methionine synthase is essential for\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            virulence, defining the biosynthetic route of this proteinogenic amino acid as a potential antifungal target. In conclusion, we provide novel insights into the nutritional requirements of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            during pathogenesis, a prerequisite to understanding and fighting infection.\n          <\/jats:p>","DOI":"10.1128\/iai.01124-15","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T03:58:49Z","timestamp":1453262329000},"page":"917-929","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant"],"prefix":"10.1128","volume":"84","author":[{"given":"Jorge","family":"Amich","sequence":"first","affiliation":[{"name":"Department of Medicine II and Center for Interdisciplinary Clinical Research, University Hospital W\u00fcrzburg, W\u00fcrzburg, Germany"},{"name":"Research Center for Infectious Diseases, Julius Maximilians University, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"D\u00fcmig","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gr\u00e1inne","family":"O'Keeffe","sequence":"additional","affiliation":[{"name":"Department of Biology, Maynooth University, Co. 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