{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:14:18Z","timestamp":1790403258783,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MCTI | Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico"},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/23289-3"],"award-info":[{"award-number":["2012\/23289-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/09167-5"],"award-info":[{"award-number":["2013\/09167-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/05073-3"],"award-info":[{"award-number":["2012\/05073-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/04391-1"],"award-info":[{"award-number":["2012\/04391-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/04860-7"],"award-info":[{"award-number":["2014\/04860-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The extraintestinal pathogen termed avian pathogenic\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            (APEC) is known to cause colibacillosis in chickens. The molecular basis of APEC pathogenesis is not fully elucidated yet. In this work, we deleted a component of the Yad gene cluster (\n            <jats:italic>yadC<\/jats:italic>\n            ) in order to understand the role of Yad in the pathogenicity of the APEC strain SCI-07.\n            <jats:italic>In vitro<\/jats:italic>\n            , the transcription level of\n            <jats:italic>yadC<\/jats:italic>\n            was upregulated at 41\u00b0C and downregulated at 22\u00b0C. The\n            <jats:italic>yadC<\/jats:italic>\n            expression\n            <jats:italic>in vivo<\/jats:italic>\n            was more pronounced in lungs than in spleen, suggesting a role in the early steps of the infection. Chicks infected with the wild-type and mutant strains presented, respectively, 80% and 50% mortality rates. The \u0394\n            <jats:italic>yadC<\/jats:italic>\n            strain presented a slightly decreased ability to adhere to HeLa cells with or without the\n            <jats:sc>d<\/jats:sc>\n            -mannose analog compared with the wild type. Real-time PCR (RT-PCR) assays showed that\n            <jats:italic>fimH<\/jats:italic>\n            was downregulated (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) and\n            <jats:italic>csgA<\/jats:italic>\n            and\n            <jats:italic>ecpA<\/jats:italic>\n            were slightly upregulated in the mutant strain, showing that\n            <jats:italic>yadC<\/jats:italic>\n            modulates expression of other fimbriae. Bacterial internalization studies showed that the \u0394\n            <jats:italic>yadC<\/jats:italic>\n            strain had a lower number of intracellular bacteria recovered from Hep-2 cells and HD11 cells than the wild-type strain (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Motility assays in soft agar demonstrated that the \u0394\n            <jats:italic>yadC<\/jats:italic>\n            strain was less motile than the wild type (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). Curiously, flagellum-associated genes were not dramatically downregulated in the \u0394\n            <jats:italic>yadC<\/jats:italic>\n            strain. Taken together, the results show that the fimbrial adhesin Yad contributes to the pathogenicity and modulates different biological characteristics of the APEC strain SCI-07.\n          <\/jats:p>","DOI":"10.1128\/iai.01138-15","type":"journal-article","created":{"date-parts":[[2015,10,27]],"date-time":"2015-10-27T02:14:06Z","timestamp":1445912046000},"page":"187-193","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Fimbria-Encoding Gene\n            <i>yadC<\/i>\n            Has a Pleiotropic Effect on Several Biological Characteristics and Plays a Role in Avian Pathogenic Escherichia coli Pathogenicity"],"prefix":"10.1128","volume":"84","author":[{"given":"Renu","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Cabrera Galv\u00e3o","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato Pariz","family":"Maluta","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana\u00edna Luisa","family":"Leite","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia Pilatti Mendes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson","family":"Nakazato","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biological Science Center, University of Londrina\u2014UEL, Londrina, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanderley","family":"Dias da Silveira","sequence":"additional","affiliation":[{"name":"Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas\u2014UNICAMP, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"299","article-title":"Avian pathogenic Escherichia coli (APEC)","volume":"30","author":"Dho-Moulin M","year":"1999","unstructured":"Dho-Moulin M, Fairbrother JM. 1999. 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