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In this study, the mRNA levels of the Dectin-1 gene were found to be decreased by 61% in alveolar macrophages (AMs) from\n                    <jats:italic>Pneumocystis<\/jats:italic>\n                    -infected mice. The expression of Dectin-1 protein on the surface of these cells was also significantly decreased. By fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization, mRNA expression levels of the transcription factor PU.1 were also found to be significantly reduced in AMs from\n                    <jats:italic>Pneumocystis<\/jats:italic>\n                    -infected mice. Electrophoretic mobility shift assay showed that PU.1 protein bound Dectin-1 gene promoter. With a luciferase reporter gene driven by the Dectin-1 gene promoter, the expression of the PU.1 gene in NIH 3T3 cells was found to enhance the luciferase activity in a dose-dependent manner. PU.1 expression knockdown by small interfering RNA (siRNA) caused a 63% decrease in Dectin-1 mRNA level and 40% decrease in protein level in AMs. Results of this study indicate that downregulation of PU.1 during\n                    <jats:italic>Pneumocystis<\/jats:italic>\n                    pneumonia leads to decreased expression of Dectin-1 in AMs.\n                  <\/jats:p>","DOI":"10.1128\/iai.01141-09","type":"journal-article","created":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T21:44:53Z","timestamp":1263246293000},"page":"1058-1065","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Downregulation of PU.1 Leads to Decreased Expression of Dectin-1 in Alveolar Macrophages during\n                    <i>Pneumocystis<\/i>\n                    Pneumonia"],"prefix":"10.1128","volume":"78","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Hung","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Ping","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoujin","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Beijing Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Lasbury","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"Durant","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Hung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana"},{"name":"Graduate Institute of Clinical Medical Science and Department of Laboratory Medicine, China Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00216.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.03.016"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5470.1439"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2008.23"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1990.tb01167.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.10.4135"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.6.1985"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.60.2.353"},{"key":"e_1_3_2_10_2","first-page":"12S","volume":"38","year":"1991","unstructured":"Garner, R. 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