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Despite widespread and efficient vaccination, there has been a world-wide resurgence of pertussis, which remains the leading cause of vaccine-preventable death in developed countries. It has been proposed that current acellular vaccines (Pa) composed of only a few bacterial proteins may be less efficacious because of vaccine-induced antigenic shifts and adaptations. To gain insight into the development of a newer generation of vaccines, we constructed a<jats:italic>Bordetella bronchiseptica<\/jats:italic>strain (LPaV) that does not express the antigenic homologs included in any of the Pa vaccines currently in use. This strain also lacks adenylate cyclase toxin, an essential virulence factor, and BipA, a surface protein. While LPaV colonized the mouse nose as efficiently as the wild-type strain, it was highly deficient in colonization of the lower respiratory tract and was attenuated in induction of inflammation and injury to the lungs. Strikingly, to our surprise, we found that in an intranasal murine challenge model, LPaV elicited cross-species protection against both<jats:italic>B. bronchiseptica<\/jats:italic>and<jats:italic>Bordetella pertussis<\/jats:italic>. Our data suggest the presence of immunogenic protective components other than those included in the pertussis vaccine. Combined with the whole-genome sequences of many<jats:italic>Bordetella<\/jats:italic>spp. that are available, the results of this study should serve as a platform for strategic development of the next generation of acellular pertussis vaccines.<\/jats:p>","DOI":"10.1128\/iai.01142-09","type":"journal-article","created":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T03:11:23Z","timestamp":1266894683000},"page":"2008-2016","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Cross-Species Protection Mediated by a<i>Bordetella bronchiseptica<\/i>Strain Lacking Antigenic Homologs Present in Acellular Pertussis Vaccines"],"prefix":"10.1128","volume":"78","author":[{"given":"Neelima","family":"Sukumar","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gina Parise","family":"Sloan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt S.","family":"Conover","sequence":"additional","affiliation":[{"name":"Program in Molecular Genetics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheraton F.","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seema","family":"Mattoo","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Pharmacology, University of California, San Diego School of Medicine, San Diego, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy D.","family":"Kock","sequence":"additional","affiliation":[{"name":"Department of Pathology\/Comparative Medicine, Wake Forest University Health Sciences, Medical Center Blvd., Winston-Salem, North Carolina 27157"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendar","family":"Deora","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Program in Molecular Genetics"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.11.020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.2460\/ajvr.1981.42.07.1130","volume":"42","year":"1981","unstructured":"Bey, R. 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