{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:53:13Z","timestamp":1791060793624,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Available vaccines against\n            <jats:italic>Brucella<\/jats:italic>\n            spp. are live attenuated\n            <jats:italic>Brucella<\/jats:italic>\n            strains. In order to engineer a better vaccine to be used in animals and humans, our laboratory aims to develop an innocuous subunit vaccine. Particularly, we are interested in the outer membrane proteins (OMPs) of\n            <jats:italic>B. abortus<\/jats:italic>\n            : Omp16 and Omp19. In this study, we assessed the use of these proteins as vaccines against\n            <jats:italic>Brucella<\/jats:italic>\n            in BALB\/c mice. Immunization with lipidated Omp16 (L-Omp16) or L-Omp19 in incomplete Freund's adjuvant (IFA) conferred significant protection against\n            <jats:italic>B. abortus<\/jats:italic>\n            infection. Vaccination with unlipidated Omp16 (U-Omp16) or U-Omp19 in IFA induced a higher degree of protection than the respective lipidated versions. Moreover, the level of protection induced after U-Omp16 or U-Omp19 immunization in IFA was similar to that elicited by live\n            <jats:italic>B. abortus<\/jats:italic>\n            S19 immunization. Flow cytometric analysis showed that immunization with U-Omp16 or U-Omp19 induced antigen-specific CD4\n            <jats:sup>+<\/jats:sup>\n            as well as CD8\n            <jats:sup>+<\/jats:sup>\n            T cells producing gamma interferon. In vivo depletion of CD4\n            <jats:sup>+<\/jats:sup>\n            or CD8\n            <jats:sup>+<\/jats:sup>\n            T cells in mice immunized with U-Omp16 or U-Omp19 plus IFA resulted in a loss of the elicited protection, indicating that both cell types are mediating immune protection. U-Omp16 or U-Omp19 vaccination induced a T helper 1 response, systemic protection in aluminum hydroxide formulation, and oral protection with cholera toxin adjuvant against\n            <jats:italic>B. abortus<\/jats:italic>\n            infection. Both immunization routes exhibited a similar degree of protection to attenuated\n            <jats:italic>Brucella<\/jats:italic>\n            vaccines (S19 and RB51, respectively). Overall these results indicate that U-Omp16 or U-Omp19 would be a useful candidate for a subunit vaccine against human and animal brucellosis.\n          <\/jats:p>","DOI":"10.1128\/iai.01151-08","type":"journal-article","created":{"date-parts":[[2008,11,3]],"date-time":"2008-11-03T20:58:59Z","timestamp":1225745939000},"page":"436-445","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":133,"title":["Immunization with Recombinant\n            <i>Brucella<\/i>\n            Species Outer Membrane Protein Omp16 or Omp19 in Adjuvant Induces Specific CD4\n            <sup>+<\/sup>\n            and CD8\n            <sup>+<\/sup>\n            T Cells as Well as Systemic and Oral Protection against\n            <i>Brucella abortus<\/i>\n            Infection"],"prefix":"10.1128","volume":"77","author":[{"given":"Karina A.","family":"Pasquevich","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia M.","family":"Estein","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunologi\u0301a, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Garci\u0301a","family":"Samartino","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Zwerdling","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena M.","family":"Coria","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"Instituto de Estudios de la Inmunidad Humoral 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