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This infection results in pathogen dissemination to distant inflammatory sites (i.e., pathogen trafficking). The alteration in chemokine-chemokine receptor expression that contributes to this pathogen trafficking function, particularly toward sites of neovascularization in humans, is unclear. To investigate this, we utilized human monocyte-derived DCs (MoDCs) and primary endothelial cells<jats:italic>in vitro<\/jats:italic>, combined with<jats:italic>ex vivo<\/jats:italic>-isolated blood mDCs and serum from chronic periodontitis subjects and healthy controls. Our results, using conditional fimbria mutants of<jats:named-content content-type=\"genus-species\">P. gingivalis<\/jats:named-content>, show that<jats:named-content content-type=\"genus-species\">P. gingivalis<\/jats:named-content>infection of MoDCs induces an angiogenic migratory profile. This profile is enhanced by expression of DC-SIGN on MoDCs and minor mfa-1 fimbriae on<jats:named-content content-type=\"genus-species\">P. gingivalis<\/jats:named-content>and is evidenced by robust upregulation of CXCR4, but not secondary lymphoid organ (SLO)-homing CCR7. This disruption of SLO-homing capacity in response to respective chemokines closely matches surface expression of CXCR4 and CCR7 and is consistent with directed MoDC migration through an endothelial monolayer.<jats:italic>Ex vivo<\/jats:italic>-isolated mDCs from the blood of chronic periodontitis subjects, but not healthy controls, expressed a similar migratory profile; moreover, sera from chronic periodontitis subjects expressed elevated levels of CXCL12. Overall, we conclude that<jats:named-content content-type=\"genus-species\">P. gingivalis<\/jats:named-content>actively \u201ccommandeers\u201d DCs by reprogramming the chemokine receptor profile, thus disrupting SLO homing, while driving migration toward inflammatory vascular sites.<\/jats:p>","DOI":"10.1128\/iai.01157-13","type":"journal-article","created":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T00:16:11Z","timestamp":1381796171000},"page":"101-111","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Secondary Lymphoid Organ Homing Phenotype of Human Myeloid Dendritic Cells Disrupted by an Intracellular Oral Pathogen"],"prefix":"10.1128","volume":"82","author":[{"given":"Brodie","family":"Miles","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Zakhary","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"El-Awady","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Scisci","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Carrion","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"O'Neill","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Rawlings","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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