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The translocated AnkB effector of the intravacuolar pathogen\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            is a bona fide F-box protein, which is localized to the cytosolic side of the\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            -containing vacuole (LCV) and is essential for intravacuolar proliferation within macrophages and amoebae. The F-box domain of AnkB interacts with the host SCF1 E3 ubiquitin ligase that triggers the decoration of the LCV with K\n            <jats:sup>48<\/jats:sup>\n            -linked polyubiquitinated proteins that are targeted for proteasomal degradation. Here we report that AnkB becomes rapidly polyubiquitinated within the host cell, and this modification is independent of the F-box domain of AnkB, indicating host-mediated polyubiquitination. We show that the AnkB effector interacts specifically with the host E3 ubiquitin ligase Trim21. Mass spectrometry analyses have shown that AnkB is modified by K\n            <jats:sup>11<\/jats:sup>\n            -linked polyubiquitination, which has no effect on its stability. This work shows the first example of K\n            <jats:sup>11<\/jats:sup>\n            -linked polyubiquitination of a bacterial effector and its interaction with the host Trim21 ubiquitin ligase.\n          <\/jats:p>","DOI":"10.1128\/iai.01165-15","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T12:29:14Z","timestamp":1445344154000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Lysine\n            <sup>11<\/sup>\n            -Linked Polyubiquitination of the AnkB F-Box Effector of Legionella pneumophila"],"prefix":"10.1128","volume":"84","author":[{"given":"William M.","family":"Bruckert","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Louisville College of Medicine, Louisville, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef","family":"Abu 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