{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:23:29Z","timestamp":1785162209326,"version":"3.55.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"NIH","award":["R01AI047997"],"award-info":[{"award-number":["R01AI047997"]}]},{"name":"NIH","award":["R01AI064537"],"award-info":[{"award-number":["R01AI064537"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We previously associated a missense mutation of the\n            <jats:italic>tc0668<\/jats:italic>\n            gene of serial\n            <jats:italic>in vitro<\/jats:italic>\n            -passaged\n            <jats:named-content content-type=\"genus-species\">Chlamydia muridarum<\/jats:named-content>\n            , a murine model of human urogenital\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , with severely attenuated disease development in the upper genital tract of female mice. Since these mutants also contained a TC0237 Q117E missense mutation that enhances their\n            <jats:italic>in vitro<\/jats:italic>\n            infectivity, an effort was made here to isolate and characterize a\n            <jats:italic>tc0668<\/jats:italic>\n            single mutant to determine its individual contribution to urogenital pathogenicity. Detailed genetic analysis of\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            passages revealed a truncated variant with a G216* nonsense mutation of the 408-amino-acid TC0668 protein that does not produce a detectable product. Intracellular growth and infectivity of\n            <jats:named-content content-type=\"genus-species\">C. muridarum in vitro<\/jats:named-content>\n            remain unaffected in the absence of TC0668. Intravaginal inoculation of the TC0668 null mutant into C3H\/HeJ mice results in a typical course of lower genital tract infection but, unlike a pathogenic isogenic control, is unable to elicit significant chronic inflammation of the oviduct and fails to induce hydrosalpinx. Thus, TC0668 is demonstrated as an important chromosome-encoded urogenital pathogenicity factor of\n            <jats:named-content content-type=\"genus-species\">C. muridarum<\/jats:named-content>\n            and the first with these characteristics to be discovered for a\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            pathogen.\n          <\/jats:p>","DOI":"10.1128\/iai.01171-15","type":"journal-article","created":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T02:59:20Z","timestamp":1448333960000},"page":"467-479","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["The Chromosome-Encoded Hypothetical Protein TC0668 Is an Upper Genital Tract Pathogenicity Factor of Chlamydia muridarum"],"prefix":"10.1128","volume":"84","author":[{"given":"Turner Allen","family":"Conrad","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Matulich","sequence":"additional","affiliation":[{"name":"Department of Biology, University of the Incarnate Word, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathon","family":"Keck","sequence":"additional","affiliation":[{"name":"Department of Biology, University of the Incarnate Word, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Beltrami","sequence":"additional","affiliation":[{"name":"Department of Biology, University of the Incarnate Word, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqun","family":"Chen","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Institute of Pathobiology, University of South China, Hengyang, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Zhou","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Institute of Pathobiology, University of South China, Hengyang, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.95.2454.49"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.79.5.497"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.6.1397"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096330"},{"key":"e_1_3_3_6_2","first-page":"834","article-title":"Prevalence of Chlamydia trachomatis genital infection among persons aged 14\u201339 years\u2014United States, 2007\u20132012","volume":"63","author":"Torrone E","year":"2014","unstructured":"Torrone E, Papp J, Weinstock H, Centers for Disease Control and Prevention. 2014. 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