{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:41:41Z","timestamp":1790710901066,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Microparticle (MP) efflux is known to be mediated by the ABCA1 protein, and the plasma level of these cell-derived MPs is elevated considerably during human malarial infection. Therefore, two polymorphisms at positions \u2212477 and \u2212320 in the promoter of the ABCA1 gene were genotyped and tested for association with the plasma MP level in four groups of malaria patients segregated according to the clinical severity, i.e., cerebral malaria (CM), multiorgan dysfunction (MOD), noncerebral severe malaria, and uncomplicated malaria (UM). The TruCount tube-based flow cytometric method was used for the exact quantification of different cell-derived MPs in patients. Polymorphisms in the ABCA1 gene promoter were analyzed by use of the PCR\/two-primer-pair method, followed by restriction fragment length polymorphism, in 428 malaria patients. The level of circulating plasma MPs was significantly higher in febrile patients with\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            infection, especially in CM patients compared to healthy individuals. The homozygous wild-type \u2212477 and \u2212320 genotype was observed to be significantly higher in patients with severe malaria. These patients also showed marked increases in the plasma MP numbers compared to UM patients. We report here for the first time an association of ABCA1 promoter polymorphisms with susceptibility to severe malaria, especially to CM and MOD, indicating the protective effect of the mutant variant of the polymorphism. We hypothesize that the \u2212477T and \u2212320G polymorphisms affect the downregulation of MP efflux and may be a predictor of organ complication during\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            malarial infections.\n          <\/jats:p>","DOI":"10.1128\/iai.01175-12","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T02:41:05Z","timestamp":1360032065000},"page":"1287-1294","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Promoter Polymorphisms in the ATP Binding Cassette Transporter Gene Influence Production of Cell-Derived Microparticles and Are Highly Associated with Susceptibility to Severe Malaria in Humans"],"prefix":"10.1128","volume":"81","author":[{"given":"Upasana","family":"Sahu","sequence":"first","affiliation":[{"name":"Division of Molecular Biology, Regional Medical Research Centre, Bhubaneswar, Orissa, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biranchi N.","family":"Mohapatra","sequence":"additional","affiliation":[{"name":"Department of Medicine, SCB Medical College, Cuttack, Orissa, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu K.","family":"Kar","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Regional Medical Research Centre, Bhubaneswar, Orissa, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoranjan","family":"Ranjit","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Regional Medical Research Centre, Bhubaneswar, Orissa, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1057","article-title":"An immunohistochemical study of the pathology of fatal malaria: evidence for widespread endothelial activation and a potential role for intercellular adhesion molecule-1 in cerebral sequestration","volume":"145","author":"Turner GD","year":"1994","unstructured":"TurnerGDMorrisonHJonesM. 1994. 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