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The primary\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            species associated with human disease are\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , which is the leading cause of both reportable bacterial sexually transmitted infections and preventable blindness, and\n            <jats:named-content content-type=\"genus-species\">C. pneumoniae<\/jats:named-content>\n            , which infects the respiratory tract and is associated with cardiovascular disease. Collectively, these pathogens are a significant source of morbidity and pose a substantial financial burden on the global economy. Past efforts to elucidate virulence mechanisms of these unique and important pathogens were largely hindered by an absence of genetic methods. Watershed studies in 2011 and 2012 demonstrated that forward and reverse genetic approaches were feasible with\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            and that shuttle vectors could be selected and maintained within the bacterium. While these breakthroughs have led to a steady expansion of the chlamydial genetic tool kit, there are still roads left to be traveled. This minireview provides a synopsis of the currently available genetic methods for\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            along with a comparison to the methods used in other obligate intracellular bacteria. Limitations and advantages of these techniques will be discussed with an eye toward the methods still needed, and how the current state of the art for genetics in obligate intracellular bacteria could direct future technological advances for\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.01186-15","type":"journal-article","created":{"date-parts":[[2015,12,15]],"date-time":"2015-12-15T04:24:41Z","timestamp":1450153481000},"page":"612-621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["A Coming of Age Story: Chlamydia in the Post-Genetic Era"],"prefix":"10.1128","volume":"84","author":[{"given":"Anna J.","family":"Hooppaw","sequence":"first","affiliation":[{"name":"Department of Microbiology, Southern Illinois University, Carbondale, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek J.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Southern Illinois University, Carbondale, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162818"},{"key":"e_1_3_2_3_2","unstructured":"Centers for Disease Control and Prevention. 2014. 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