{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:24:11Z","timestamp":1793075051252,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"NIH","award":["1 R01 AI112595-01"],"award-info":[{"award-number":["1 R01 AI112595-01"]}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGF 1106401"],"award-info":[{"award-number":["DGF 1106401"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Myosins are a family of actin-based motor proteins found in many organisms and are categorized into classes based on their structures. Class II and V myosins are known to be important for critical cellular processes, including cytokinesis, endocytosis, exocytosis, and organelle trafficking, in the model fungi\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Aspergillus nidulans<\/jats:named-content>\n            . However, the roles of myosins in the growth and virulence of the pathogen\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            are unknown. We constructed single- and double-deletion strains of the class II and class V myosins in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            and found that while the class II myosin (\n            <jats:italic>myoB<\/jats:italic>\n            ) is dispensable for growth, the class V myosin (\n            <jats:italic>myoE<\/jats:italic>\n            ) is required for proper hyphal extension; deletion of\n            <jats:italic>myoE<\/jats:italic>\n            resulted in hyperbranching and loss of hyphal polarity. Both\n            <jats:italic>myoB<\/jats:italic>\n            and\n            <jats:italic>myoE<\/jats:italic>\n            are necessary for proper septation, conidiation, and conidial germination, but only\n            <jats:italic>myoB<\/jats:italic>\n            is required for conidial viability. Infection with the \u0394\n            <jats:italic>myoE<\/jats:italic>\n            strain in the invertebrate\n            <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n            model and also in a persistently immunosuppressed murine model of invasive aspergillosis resulted in hypovirulence, while analysis of bronchoalveolar lavage fluid revealed that tumor necrosis factor alpha (TNF-\u03b1) release and cellular infiltration were similar compared to those of the wild-type strain. The \u0394\n            <jats:italic>myoE<\/jats:italic>\n            strain showed fungal growth in the murine lung, while the \u0394\n            <jats:italic>myoB<\/jats:italic>\n            strain exhibited little fungal burden, most likely due to the reduced conidial viability. These results show, for the first time, the important role these cytoskeletal components play in the growth of and disease caused by a known pathogen, prompting future studies to understand their regulation and potential targeting for novel antifungal therapies.\n          <\/jats:p>","DOI":"10.1128\/iai.01190-15","type":"journal-article","created":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T03:38:27Z","timestamp":1457408307000},"page":"1556-1564","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis"],"prefix":"10.1128","volume":"84","author":[{"given":"Hilary","family":"Renshaw","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 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