{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T08:45:09Z","timestamp":1791017109321,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            High numbers of lactobacilli in the vaginal tract have been correlated with a decreased risk of infection by the sexually transmitted pathogen\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            . We have previously shown that\n            <jats:italic>Lactobacillus jensenii<\/jats:italic>\n            , one of the most prevalent microorganisms in the healthy human vaginal tract, can inhibit gonococcal adherence to epithelial cells in culture. Here we examined the role of the epithelial cells and the components of\n            <jats:italic>L. jensenii<\/jats:italic>\n            involved in the inhibition of gonococcal adherence.\n            <jats:italic>L. jensenii<\/jats:italic>\n            inhibited the adherence of gonococci to glutaraldehyde-fixed epithelial cells like it inhibited the adherence of gonococci to live epithelial cells, suggesting that the epithelial cells do not need to be metabolically active for the inhibition to occur. In addition, methanol-fixed\n            <jats:italic>L. jensenii<\/jats:italic>\n            inhibited gonococcal adherence to live epithelial cells, indicating that\n            <jats:italic>L. jensenii<\/jats:italic>\n            uses a constitutive component to inhibit gonococcal interactions with epithelial cells. Proteinase K treatment of methanol-fixed lactobacilli eliminated the inhibitory effect, suggesting that the inhibitory component contains protein. Released surface components (RSC) isolated from\n            <jats:italic>L. jensenii<\/jats:italic>\n            were found to contain at least two inhibitory components, both of which are protease sensitive. Using anion-exchange and size exclusion chromatography, an inhibitory protein which exhibits significant similarity to the enzyme enolase was isolated. A recombinant His\n            <jats:sub>6<\/jats:sub>\n            -tagged version of this protein was subsequently produced and shown to inhibit gonococcal adherence to epithelial cells in a dose-dependent manner.\n          <\/jats:p>","DOI":"10.1128\/iai.01200-09","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T03:30:30Z","timestamp":1271129430000},"page":"3103-3111","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["<i>Lactobacillus jensenii<\/i>\n            Surface-Associated Proteins Inhibit\n            <i>Neisseria gonorrhoeae<\/i>\n            Adherence to Epithelial Cells"],"prefix":"10.1128","volume":"78","author":[{"given":"Rachel R.","family":"Spurbeck","sequence":"first","affiliation":[{"name":"Genetics Program, Michigan State University, East Lansing, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy Grove","family":"Arvidson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics"},{"name":"Genetics Program, Michigan State University, East Lansing, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria 2001"},{"key":"e_1_3_2_3_2","first-page":"332","volume":"56","year":"2007","unstructured":"Anonymous. 2007. Update to CDC's sexually transmitted diseases treatment guidelines, 2006: fluoroquinolones no longer recommended for treatment of gonococcal infections. MMWR Morb. Mortal. Wkly. Rep.56:332-336.","journal-title":"MMWR Morb. Mortal. Wkly. 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Inhibition of Escherichia coli O157:H7 attachment by interactions between lactic acid bacteria and intestinal epithelial cells. J. Microbiol. Biotechnol.18:1278-1285.","journal-title":"J. Microbiol. 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The reproductive system and its indigenous microbiota, p. 206-250. InMicrobial inhabitants of humans: their ecology and role in health and disease. Cambridge University Press, New York, NY.","journal-title":"Microbial inhabitants of humans: their ecology and role in health and disease."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.12"}],"updated-by":[{"DOI":"10.1128\/iai.00789-12","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2012,9,11]],"date-time":"2012-09-11T00:00:00Z","timestamp":1347321600000}}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01200-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01200-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:47:43Z","timestamp":1646473663000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01200-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1128\/IAI.01200-09"],"URL":"https:\/\/doi.org\/10.1128\/iai.01200-09","relation":{"correction":[{"id-type":"doi","id":"10.1128\/IAI.00789-12","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]},"assertion":[{"value":"2009-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}