{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:48:44Z","timestamp":1767167324517,"version":"build-2238731810"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We assessed the efficacy of a fusion protein consisting of the 25-kDa antigenic region of\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    hemagglutinin A and the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    maltose-binding protein (25k-hagA-MBP) as a nasal vaccine for the prevention of oral infection with\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    . Nasal immunization with 25k-hagA-MBP induced high levels of 25k-hagA-specific serum IgG, serum IgA, and salivary IgA antibodies in a Toll-like receptor 4 (TLR4)-dependent manner. These antibody responses were maintained for at least 1 year after immunization. Analysis of cytokine responses showed that nasal administration of 25k-hagA-MBP induced antigen-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells producing interleukin 4 (IL-4) and IL-5, but not gamma interferon (IFN-\u03b3), in the spleen and cervical lymph nodes (CLNs). Furthermore, increased numbers of CD11c\n                    <jats:sup>+<\/jats:sup>\n                    CD8\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    , but not CD11c\n                    <jats:sup>+<\/jats:sup>\n                    CD11b\n                    <jats:sup>+<\/jats:sup>\n                    or CD11c\n                    <jats:sup>+<\/jats:sup>\n                    B220\n                    <jats:sup>+<\/jats:sup>\n                    , dendritic cells with upregulated expression of CD80, CD86, CD40, and major histocompatibility complex class II (MHC II) molecules were noted in the spleen, CLNs, and nasopharynx-associated lymphoreticular tissues (NALT). Interestingly, when 25k-hagA-MBP or cholera toxin (CT) was given intranasally to enable examination of their presence in neuronal tissues, the amounts of 25k-hagA-MBP were significantly lower than those of CT. Importantly, mice given 25k-hagA-MBP nasally showed a significant reduction in alveolar bone loss caused by oral infection with\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    , even 1 year after the immunization. These results suggest that 25k-hagA-MBP administered nasally would be an effective and safe mucosal vaccine against\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    infection and may be an important tool for the prevention of chronic periodontitis in humans.\n                  <\/jats:p>","DOI":"10.1128\/iai.01203-10","type":"journal-article","created":{"date-parts":[[2010,11,30]],"date-time":"2010-11-30T00:12:27Z","timestamp":1291075947000},"page":"895-904","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Nasal Immunization with a Fusion Protein Consisting of the Hemagglutinin A Antigenic Region and the Maltose-Binding Protein Elicits CD11c\n                    <sup>+<\/sup>\n                    CD8\n                    <sup>+<\/sup>\n                    Dendritic Cells for Induced Long-Term Protective Immunity"],"prefix":"10.1128","volume":"79","author":[{"given":"Yuan","family":"Du","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"},{"name":"Stomatology College of Tianjin Medical University, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Hashizume","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Kurita-Ochiai","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Yuzawa","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Abiko","sequence":"additional","affiliation":[{"name":"Molecular Biology and Biochemistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(94)90055-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.10s.1123"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021908"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.1.204-229.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2540"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.6.1932-1940.1991"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88874-5"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3784-3791.1999"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.306.6879.688"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.45.145"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00486-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.45201"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.06.091"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000129769.17895.F0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.51"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00412-6"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.10.4000-4007.1996"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01145-07"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(92)90102-7"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199810)28:10<3346::AID-IMMU3346>3.0.CO;2-P"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1213"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00162.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2001.6.1.125"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.2.229"},{"key":"e_1_3_2_26_2","first-page":"1","volume":"20","year":"1994","unstructured":"Iwasaki, S., M. Hayakawa, and H. Takiguchi. 1994. Gene cloning of the fusion protein of Porphyromonas gingivalis major antigenic region of 200-kDa antigen and maltose binding protein. Nihon Univ. J. Oral Sci. 20:1-12.","journal-title":"Nihon Univ. J. Oral Sci."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.2.803"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3612"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.142"},{"key":"e_1_3_2_30_2","first-page":"494","volume":"97","year":"1989","unstructured":"Klausen, B., R. T. Evans, and C. Sfintescu. 1989. Two complementary methods of assessing periodontal bone level in rats. Scand. J. Dent. Res. 97:494-499.","journal-title":"Scand. J. Dent. Res."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.732-739.2000"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2008.07.008"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2001.00390.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000082462.26258.FE"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.18.10078-10087.2003"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00339.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.10.036"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb01040.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00176.x"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(98)80147-1"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030595"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.8.2528-2537.2003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02106.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1989.tb00238.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1995.tb00160.x"},{"key":"e_1_3_2_46_2","first-page":"21","volume":"23","year":"2002","unstructured":"Reddy, M. S. 2002. Oral osteoporosis: is there an association between periodontitis and osteoporosis? Compend. Contin. Educ. Dent. 23:21-28.","journal-title":"Compend. Contin. Educ. Dent."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.347-352.1998"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:15:1:51"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1541-1545.1997"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.8.5012"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00870.x"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.420"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.655"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.48.227"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.9.4778"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)90033-7"},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.4049\/jimmunol.161.8.4115","volume":"161","year":"1998","unstructured":"Yamamoto, M., et al. 1998. A nontoxic adjuvant for mucosal immunity to pneumococcal surface protein A. J. Immunol. 161:4115-4121.","journal-title":"J. Immunol."},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.2.640-644.1997"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2001.00883.x"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1996.tb21115.x"},{"key":"e_1_3_2_61_2","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.4049\/jimmunol.162.6.3559","volume":"162","year":"1999","unstructured":"Yanagita, M., et al. 1999. Nasopharyngeal-associated lymphoreticular tissue (NALT) immunity: fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production. J. Immunol. 162:3559-3565.","journal-title":"J. Immunol."}],"updated-by":[{"DOI":"10.1128\/iai.01023-15","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000}}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01203-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01203-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,4]],"date-time":"2023-06-04T05:34:57Z","timestamp":1685856897000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01203-10"}},"subtitle":[],"editor":[{"given":"S. R.","family":"Blanke","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":60,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1128\/IAI.01203-10"],"URL":"https:\/\/doi.org\/10.1128\/iai.01203-10","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"2010-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}