{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T01:54:50Z","timestamp":1773107690134,"version":"3.50.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI072606"],"award-info":[{"award-number":["R01AI072606"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["P30GM110760"],"award-info":[{"award-number":["P30GM110760"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Scientific analysis of the genus\n            <jats:named-content content-type=\"genus-species\">Rickettsia<\/jats:named-content>\n            is undergoing a rapid period of change with the emergence of viable genetic tools. The development of these tools for the mutagenesis of pathogenic bacteria will permit forward genetic analysis of\n            <jats:named-content content-type=\"genus-species\">Rickettsia<\/jats:named-content>\n            pathogenesis. Despite these advances, uncertainty still remains regarding the use of plasmids to study these bacteria in\n            <jats:italic>in vivo<\/jats:italic>\n            mammalian models of infection, namely, the potential for virulence changes associated with the presence of extrachromosomal DNA and nonselective persistence of plasmids in mammalian models of infection. Here, we describe the transformation of\n            <jats:named-content content-type=\"genus-species\">Rickettsia conorii<\/jats:named-content>\n            Malish 7 with the plasmid pRam18dRGA[AmTrCh]. Transformed\n            <jats:named-content content-type=\"genus-species\">R. conorii<\/jats:named-content>\n            stably maintains this plasmid in infected cell cultures, expresses the encoded fluorescent proteins, and exhibits growth kinetics in cell culture similar to those of nontransformed\n            <jats:named-content content-type=\"genus-species\">R. conorii<\/jats:named-content>\n            . Using a well-established murine model of fatal Mediterranean spotted fever, we demonstrate that\n            <jats:named-content content-type=\"genus-species\">R. conorii<\/jats:named-content>\n            (pRam18dRGA[AmTrCh]) elicits the same fatal outcomes in animals as its untransformed counterpart and, importantly, maintains the plasmid throughout infection in the absence of selective antibiotic pressure. Interestingly, plasmid-transformed\n            <jats:named-content content-type=\"genus-species\">R. conorii<\/jats:named-content>\n            was readily observed both in endothelial cells and within circulating leukocytes. Together, our data demonstrate that the presence of an extrachromosomal DNA element in a pathogenic rickettsial species does not affect either\n            <jats:italic>in vitro<\/jats:italic>\n            proliferation or\n            <jats:italic>in vivo<\/jats:italic>\n            infectivity in models of disease and that plasmids such as pRam18dRGA[AmTrCh] are valuable tools for the further genetic manipulation of pathogenic rickettsiae.\n          <\/jats:p>","DOI":"10.1128\/iai.01205-15","type":"journal-article","created":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T03:48:58Z","timestamp":1452570538000},"page":"790-797","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection"],"prefix":"10.1128","volume":"84","author":[{"given":"Sean P.","family":"Riley","sequence":"first","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail I.","family":"Fish","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Garza","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaikhushroo H.","family":"Banajee","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma K.","family":"Harris","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"del Piero","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan J.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Vector Borne Disease Laboratories, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA"},{"name":"Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, 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