{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:34:39Z","timestamp":1770910479646,"version":"3.50.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the thermodimorphic fungus\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Paracoccidioides brasiliensis<\/jats:named-content>\n            . Leukotrienes and lipoxins are lipid mediators produced after 5-lipoxygenase (5-LO) activation that exhibit pro- and anti-inflammatory roles, respectively. Here, we have investigated the contribution of 5-LO enzymatic activity in PCM using an experimental model of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. brasiliensis<\/jats:named-content>\n            infection. B6.129 wild-type (B6.129) and 5-LO-deficient (5-LO\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            ) mice were intravenously inoculated with a virulent strain of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. brasiliensis<\/jats:named-content>\n            (Pb18), and the survival rate of the infected mice was investigated on different days after yeast infection. 5-LO\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice exhibited an increased survival rate associated with a decreased number of CFU. The resistance of 5-LO\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            during PCM was associated with augmented nitric oxide (NO) production and the formation of compact granulomas. In addition, the absence of 5-LO was associated with a diminished number of CD4\n            <jats:sup>+<\/jats:sup>\n            CD25\n            <jats:sup>+<\/jats:sup>\n            regulatory T cells, higher levels of gamma interferon and interleukin-12, and increased T-bet (a T-box transcription factor that directs Th1 lineage commitment) mRNA levels in the lungs. Taken together, our results show for the first time that 5-LO enzymatic activity increases susceptibility to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. brasiliensis<\/jats:named-content>\n            , suggesting that this pathway may be a potential target for therapeutic intervention during PCM.\n          <\/jats:p>","DOI":"10.1128\/iai.01209-12","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T02:41:05Z","timestamp":1360032065000},"page":"1256-1266","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["5-Lipoxygenase Activity Increases Susceptibility to Experimental Paracoccidioides brasiliensis Infection"],"prefix":"10.1128","volume":"81","author":[{"given":"Fabrine Sales Massafera","family":"Trist\u00e3o","sequence":"first","affiliation":[{"name":"Departamentos de Bioqu\u00edmica e Imunologia, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Agostini","family":"Rocha","sequence":"additional","affiliation":[{"name":"Departamentos de Bioqu\u00edmica e Imunologia, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Moreira","sequence":"additional","affiliation":[{"name":"Departamentos de Bioqu\u00edmica e Imunologia, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Queiroz","family":"Cunha","sequence":"additional","affiliation":[{"name":"Farmacologia e, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Antonio","family":"Rossi","sequence":"additional","affiliation":[{"name":"Patologia, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Santana","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamentos de Bioqu\u00edmica e Imunologia, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.3.233.241"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2007.09.007"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2002000500037"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2007.11.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.9.5811"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.5.3049"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903851106"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141647"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-3278(03)00070-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199009063231006"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni745"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23949"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219380000251"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219280000331"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00683967"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.12.050"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-007-9040-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6451927"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1107738"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060271"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63686-3"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.5.1455"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.9.4593"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1992.394"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801982762"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.01.006"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-11-3411"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.607"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2005.05.018"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801895444"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021423"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86821985000200006"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.03.016"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021183"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.11.6533"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.2741\/2692"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902009"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.39"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.5.1209"}],"container-title":["Infection 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