{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:28:24Z","timestamp":1791052104945,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The recombinant protective antigen (rPA) of\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            is a promising anthrax vaccine. We compared serum immunoglobulin G levels and toxin-neutralizing antibody titers in rabbits following delivery of various doses of vaccine by microneedle-based intradermal (i.d.) delivery or intramuscular (i.m.) injection using conventional needles. Intradermal delivery required less antigen to induce levels of antibody similar to those produced via i.m. injection during the first 2 weeks following primary and booster inoculation. This dose-sparing effect was less evident at the later stages of the immune response. Rabbits immunized i.d. with 10 \u03bcg of rPA displayed 100% protection from aerosol spore challenge, while i.m. injection of the same dose provided slightly lower protection (71%). Groups immunized with lower antigen doses were partially protected (13 to 29%) regardless of the mode of administration. Overall, our results suggest rPA formulated with aluminum adjuvant and administered to the skin by a microneedle-based device is as efficacious as i.m. vaccination.\n          <\/jats:p>","DOI":"10.1128\/iai.01210-06","type":"journal-article","created":{"date-parts":[[2006,11,21]],"date-time":"2006-11-21T01:05:37Z","timestamp":1164071137000},"page":"6806-6810","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Microneedle-Based Intradermal Delivery of the Anthrax Recombinant Protective Antigen Vaccine"],"prefix":"10.1128","volume":"74","author":[{"given":"John A.","family":"Mikszta","sequence":"first","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"John P.","family":"Dekker","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noel G.","family":"Harvey","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl H.","family":"Dean","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Brittingham","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Huang","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent J.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"BD Technologies, Research Triangle Park, North Carolina 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly","family":"Dyas","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad J.","family":"Roy","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Ulrich","sequence":"additional","affiliation":[{"name":"U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043555"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000012159.20895.5b"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.1.3.1797"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2022-2028.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.5.1239"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.05.044"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0906.020728"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)80112-6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043540"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/422694"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00118-9"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.07.004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.12.028"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.6.1606-1613.1990"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0402-415"},{"key":"e_1_3_2_18_2","first-page":"309","year":"2006","unstructured":"Mikszta, J. A., M. I. Haider, and R. J. Pettis. 2006. Microneedles for drug and vaccine delivery. When will the dream become a reality? p. 309-325. In J. Wille (ed.), Skin delivery systems. Transdermals, dermatologicals, and cosmetic actives. Blackwell Press, Ames, Iowa.","journal-title":"Skin delivery systems"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/426865"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1654(199804\/06)8:2<97::AID-RMV201>3.0.CO;2-9"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/497266"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00234-1"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/502012"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5978-5987.2005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01210-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01210-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:18:39Z","timestamp":1646461119000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01210-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1128\/IAI.01210-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01210-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12]]},"assertion":[{"value":"2006-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}