{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:37:49Z","timestamp":1791095869157,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    There is ample evidence that\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    capsular polysaccharide (CP) promotes virulence. Loss of capsule expression, however, may lead to\n                    <jats:italic>S. aureus<\/jats:italic>\n                    persistence in a chronically infected host. This study was conducted to determine the relative prevalence of nonencapsulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    in patients with chronic and acute osteomyelitis. Only 76\/118 (64%)\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates from patients with osteomyelitis expressed CP, whereas all 50 isolates from blood cultures of patients with infections other than osteoarticular infections expressed CP (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0001). A significantly higher prevalence of nonencapsulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    was found in patients with chronic osteomyelitis (53%) than in those with acute osteomyelitis (21%) (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0046).\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates obtained from multiple specimens from five of six patients with chronic osteomyelitis exhibited phenotypic (expression of CP, \u03b1-hemolysin, \u03b2-hemolysin, slime, and the small-colony variant phenotype) and\/or genotypic (pulsed-field gel electrophoresis and\n                    <jats:italic>spa<\/jats:italic>\n                    typing) differences. Nonencapsulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    was recovered from at least one specimen from each chronic osteomyelitis patient. Fourteen isolates obtained from two patients with acute osteomyelitis were indistinguishable from each other within each group, and all produced CP5. In conclusion, we demonstrated that nonencapsulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    is more frequently isolated from patients with chronic osteomyelitis than from those with acute osteomyelitis, suggesting that loss of CP expression may be advantageous to\n                    <jats:italic>S. aureus<\/jats:italic>\n                    during chronic infection. Our findings on multiple\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates from individual patients allow us to suggest that selection of nonencapsulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    is likely to have occurred in the patient during long-term bone infection.\n                  <\/jats:p>","DOI":"10.1128\/iai.01214-08","type":"journal-article","created":{"date-parts":[[2009,3,9]],"date-time":"2009-03-09T20:55:09Z","timestamp":1236632109000},"page":"1968-1975","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Capsule Expression and Genotypic Differences among\n                    <i>Staphylococcus aureus<\/i>\n                    Isolates from Patients with Chronic or Acute Osteomyelitis"],"prefix":"10.1128","volume":"77","author":[{"given":"Santiago M.","family":"Lattar","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena P. N.","family":"Tuchscherr","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital of Mu\u0308nster, Mu\u0308nster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto L.","family":"Caccuri","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Centro\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital of Mu\u0308nster, Mu\u0308nster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio A.","family":"Alonso","sequence":"additional","affiliation":[{"name":"Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Barberis","sequence":"additional","affiliation":[{"name":"Hospital de Cli\u0301nicas Jose\u0301 de San Marti\u0301n, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Miranda","sequence":"additional","affiliation":[{"name":"Policli\u0301nico Bancario, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda R.","family":"Buzzola","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"von Eiff","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital of Mu\u0308nster, Mu\u0308nster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel O.","family":"Sordelli","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"},{"name":"CONICET, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00171-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.2932-2939.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.4.1033"},{"key":"e_1_3_2_5_2","first-page":"384","year":"2003","unstructured":"Bannerman, T. L. 2003. Staphylococcus, Micrococcus, and other catalase-positive cocci that grow aerobically, p. 384-404. In P. R. Murray (ed.), Manual of clinical microbiology, 8th ed. ASM Press, Washington, DC.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407460101"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1654-1660.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2003.12.007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01215-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3928\/0147-7447-19841001-07"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04978.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.2106\/00004623-199510000-00015","volume":"77","year":"1995","unstructured":"Garvin, K. L., and A. D. Hanssen. 1995. Current concepts review: infection after total hip arthroplasty. J. Bone Jt. Surg. Br. Vol.77A:1576-1588.","journal-title":"J. Bone Jt. Surg. Br. Vol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2007.01423.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(00)75073-9"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/514061"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.3.526-530.1987"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/515238"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4424-4427.2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5001-5006.1999"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.792-799.2004"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.12.2612-2615.1990"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.5.1853-1858.1993"},{"key":"e_1_3_2_23_2","first-page":"456","year":"2006","unstructured":"Lee, C. Y., and J. C. Lee. 2006. Staphylococcal capsule, p. 456-463. In V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood (ed.), Gram-positive pathogens. ASM Press, Washington, DC.","journal-title":"Gram-positive pathogens."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.618-623.1998"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5419.1523"},{"key":"e_1_3_2_26_2","first-page":"2321","year":"2005","unstructured":"Moreillon, P., Y. I. Que, and M. P. Glauser. 2005. Staphylococcus aureus, p. 2321-2351. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious disease. Elsevier-Churchill Livingstone, Philadelphia, PA.","journal-title":"Principles and practice of infectious disease."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.218-234.2004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1989.tb00262.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.3.275-278.1997"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.1.95"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1384"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00137-8"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.3928\/0147-7447-20040601-12"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.846-850.2000"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.2.407-415.1994"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.7932-7937.2005"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00747-07"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00874-08"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1086\/516962"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.15.4706-4712.1997"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01214-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01214-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:56:43Z","timestamp":1646449003000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01214-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,5]]}},"alternative-id":["10.1128\/IAI.01214-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.01214-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5]]},"assertion":[{"value":"2008-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}