{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:18:21Z","timestamp":1787170701585,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We have previously shown that one of the minimal active regions of statherin, a human salivary protein, for binding to\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    is a YQPVPE amino acid sequence. In this study, we identified the FomA protein of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    , which is responsible for binding to the statherin-derived YQPVPE peptide. Overlay analysis showed that a 40-kDa protein of the\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    cell envelope (40-kDa CE) specifically bound to the YQPVPE peptide. The equilibrium association constant between the affinity-purified 40-kDa CE and the YQPVPE peptide was 4.30 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    . Further, the purity and amino acid sequence analyses of the purified 40-kDa CE revealed approximately 98.7% (wt\/wt) purity and a high degree of homology with FomA, a major porin protein of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    . Thus, a FomA-deficient mutant failed to bind to the YQPVPE peptide. In addition, increased levels of a FomA-specific mucosal IgA antibody (Ab) and plasma IgG and IgA Abs were seen only in mice immunized nasally with cholera toxin (CT) and the purified 40-kDa FomA protein. Interestingly, saliva from mice that received FomA plus CT as a mucosal adjuvant nasally prevented\n                    <jats:italic>in vitro<\/jats:italic>\n                    binding of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    to statherin-coated polyvinyl chloride plates. Taken together, these results suggest that induction of specific immunity to the 40-kDa FomA protein of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    , which specifically binds to the statherin-derived peptide, may be an effective tool for preventing the formation of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    biofilms in the oral cavity.\n                  <\/jats:p>","DOI":"10.1128\/iai.01224-09","type":"journal-article","created":{"date-parts":[[2009,12,14]],"date-time":"2009-12-14T21:47:48Z","timestamp":1260827268000},"page":"1185-1192","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["<i>Fusobacterium nucleatum<\/i>\n                    Envelope Protein FomA Is Immunogenic and Binds to the Salivary Statherin-Derived Peptide"],"prefix":"10.1128","volume":"78","author":[{"given":"Hidetaka","family":"Nakagaki","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Sekine","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Terao","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Microbiology, Graduate School of Dentistry, Osaka University, Osaka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Toe","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneo","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiro-O","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetada","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Microbiology, Graduate School of Dentistry, Osaka University, Osaka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Shizukuishi","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohtaro","family":"Fujihashi","sequence":"additional","affiliation":[{"name":"Immunobiology Vaccine Center, Research Institute of Oral Health, Department of Pediatric Dentistry, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry"},{"name":"Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan"},{"name":"Immunobiology Vaccine Center, Research Institute of Oral Health, Department of Pediatric Dentistry, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.8.3372-3380.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.10.4249-4254.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2399-2405.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4729-4732.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp048006"},{"key":"e_1_3_2_7_2","first-page":"475","volume":"22","year":"1949","unstructured":"Edman, P. 1949. 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