{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:08:54Z","timestamp":1790665734545,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            , the causative agent of melioidosis, is an important human pathogen in Southeast Asia and northern Australia for which a vaccine is unavailable. A panel of 892 double signature-tagged mutants was screened for virulence using an intranasal BALB\/c mouse model of infection. A novel DNA tag microarray identified 33 mutants as being attenuated in spleens, while 6 were attenuated in both lungs and spleens. The transposon insertion sites in spleen-attenuated mutants revealed genes involved in several stages of capsular polysaccharide biosynthesis and DNA replication and repair, a putative oxidoreductase, ABC transporters, and a lipoprotein that may be important in intercellular spreading. The six mutants identified as missing in both lungs and spleens were found to have insertions in\n            <jats:italic>recA<\/jats:italic>\n            involved in the SOS response and DNA repair; putative auxotrophs of leucine, threonine,\n            <jats:italic>p<\/jats:italic>\n            -aminobenzoic acid, and a mutant with an insertion in\n            <jats:italic>aroB<\/jats:italic>\n            causing auxotrophy for aromatic compounds were also found. Murine challenge studies revealed partial protection in BALB\/c mice vaccinated with the\n            <jats:italic>aroB<\/jats:italic>\n            mutant. The refined signature-tagged mutagenesis approach developed in this study was used to efficiently identify attenuating mutants from this highly pathogenic species and could be applied to other organisms.\n          <\/jats:p>","DOI":"10.1128\/iai.01240-06","type":"journal-article","created":{"date-parts":[[2006,12,23]],"date-time":"2006-12-23T01:39:53Z","timestamp":1166837993000},"page":"1186-1195","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Development of Signature-Tagged Mutagenesis in\n            <i>Burkholderia pseudomallei<\/i>\n            To Identify Genes Important in Survival and Pathogenesis"],"prefix":"10.1128","volume":"75","author":[{"given":"J.","family":"Cuccui","sequence":"first","affiliation":[{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Keppel Street, London WC1E 7HT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Easton","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Keppel Street, London WC1E 7HT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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