{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:09:23Z","timestamp":1791428963037,"version":"4.3.3"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bacillus anthracis<\/jats:italic>\n                    is the causative agent of anthrax, a disease that affects wildlife, livestock, and humans. Protection against anthrax is primarily afforded by immunity to the\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    protective antigen (PA), particularly PA domains 4 and 1. To further the development of an orally delivered human vaccine for mass vaccination against anthrax, we produced\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovar Typhimurium expressing full-length PA, PA domains 1 and 4, or PA domain 4 using codon-optimized PA DNA fused to the\n                    <jats:italic>S. enterica<\/jats:italic>\n                    serovar Typhi ClyA and under the control of the\n                    <jats:italic>ompC<\/jats:italic>\n                    promoter. Oral immunization of A\/J mice with\n                    <jats:italic>Salmonella<\/jats:italic>\n                    expressing full-length PA protected five of six mice against a challenge with 10\n                    <jats:sup>5<\/jats:sup>\n                    CFU of aerosolized\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    STI spores, whereas\n                    <jats:italic>Salmonella<\/jats:italic>\n                    expressing PA domains 1 and 4 provided only 25% protection (two of eight mice), and\n                    <jats:italic>Salmonella<\/jats:italic>\n                    expressing PA domain 4 or a\n                    <jats:italic>Salmonella<\/jats:italic>\n                    -only control afforded no measurable protection. However, a purified recombinant fusion protein of domains 1 and 4 provided 100% protection, and purified recombinant 4 provided protection in three of eight immunized mice. Thus, we demonstrate for the first time the efficacy of an oral\n                    <jats:italic>S. enterica<\/jats:italic>\n                    -based vaccine against aerosolized\n                    <jats:italic>B. anthracis<\/jats:italic>\n                    spores.\n                  <\/jats:p>","DOI":"10.1128\/iai.01242-06","type":"journal-article","created":{"date-parts":[[2006,12,4]],"date-time":"2006-12-04T20:33:53Z","timestamp":1165264433000},"page":"1827-1834","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Oral Administration of a\n                    <i>Salmonella enterica<\/i>\n                    -Based Vaccine Expressing\n                    <i>Bacillus anthracis<\/i>\n                    Protective Antigen Confers Protection against Aerosolized\n                    <i>B. anthracis<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Margaret G. M.","family":"Stokes","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"},{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan N.","family":"Neeson","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Galen","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development, School of Medicine, University of Maryland, Baltimore, Maryland 21201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola J.","family":"Walker","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Stagg","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic C.","family":"Jenner","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne E.","family":"Thwaite","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Nataro","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development, School of Medicine, University of Maryland, Baltimore, Maryland 21201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie W. J.","family":"Baillie","sequence":"additional","affiliation":[{"name":"University of Maryland Biotechnology Institute, University of Maryland, Baltimore, Maryland 21201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen S.","family":"Atkins","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01498.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.24.9415"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(92)90117-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)90148-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"Duc L. H. H. A. Hong H. S. Atkins H. C. Flick-Smith Z. Durrani S. Rijpkema R. W. Titball and S. M. Cutting. 2007. Immunization against anthrax using Bacillus subtilis spores expressing the anthrax protective antigen. Vaccine 25 : 346-355.","DOI":"10.1016\/j.vaccine.2006.07.037"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1653-1656.2002"},{"key":"e_1_3_2_8_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(96)00227-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02096-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6424-6433.1999"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7096-7106.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00619.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3831-3836.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/131.5.553"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1947.150.1.70"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400015801"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400019422"},{"key":"e_1_3_2_19_2","first-page":"728","volume":"32","year":"2002","unstructured":"Hobert, O. 2002. PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic Caenorhabditis elegans. Bio Techniques32:728-730.","journal-title":"Bio Techniques"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00152686"},{"key":"e_1_3_2_21_2","first-page":"543","year":"1995","unstructured":"Leppla, S. H. 1995. Anthrax toxins, p. 543-572. In J. Moss, B. Iglewski, M. Vaughan, and A. Tu (ed.), Bacterial toxins and virulence factors in disease. Marcel Dekker, Inc., New York, NY.","journal-title":"Bacterial toxins and virulence factors in disease"},{"key":"e_1_3_2_22_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-8502(73)90006-2"},{"key":"e_1_3_2_24_2","first-page":"287","volume":"14","year":"1957","unstructured":"May, K. R., and G. H. Harper. 1957. The efficacy of various liquid impinger samplers in bacterial aerosols. Br. Ind. Med.14:287-297.","journal-title":"Br. Ind. Med."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00268-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.1998.00274.x"},{"key":"e_1_3_2_27_2","first-page":"1369","year":"1995","unstructured":"Pezard, C., M. Weber, J.-C. Sirard, P. Berche, and M. Mock. 1995. Protective immunity induced by Bacillus anthracis toxin-deficient strains. Infect. Immun.1369-1372.","journal-title":"Infect. Immun."},{"key":"e_1_3_2_28_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/423937"},{"key":"e_1_3_2_30_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.227-234.2002"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00754-2"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.3.795-800.1986"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01242-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01242-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:02:12Z","timestamp":1646445732000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01242-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/IAI.01242-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01242-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}