{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:20Z","timestamp":1791343520046,"version":"4.3.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Most members of the genus\n            <jats:italic>Brucella<\/jats:italic>\n            show strong urease activity. However, the role of this enzyme in the pathogenesis of\n            <jats:italic>Brucella<\/jats:italic>\n            infections is poorly understood. We isolated several Tn\n            <jats:italic>5<\/jats:italic>\n            insertion mutants deficient in urease activity from\n            <jats:italic>Brucella abortus<\/jats:italic>\n            strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated\n            <jats:italic>ure1<\/jats:italic>\n            , revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene (\n            <jats:italic>cobT<\/jats:italic>\n            ) was identified, and inactivation of this gene affected urease activity in\n            <jats:italic>Brucella<\/jats:italic>\n            . Subsequent analysis of the previously described sequences of the genomes of\n            <jats:italic>Brucella<\/jats:italic>\n            spp. revealed the presence of a second urease cluster,\n            <jats:italic>ure2<\/jats:italic>\n            , in all them. The\n            <jats:italic>ure2<\/jats:italic>\n            locus was apparently inactive in\n            <jats:italic>B. abortus<\/jats:italic>\n            2308. Urease-deficient mutants were used to evaluate the role of urease in\n            <jats:italic>Brucella<\/jats:italic>\n            pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis.\n          <\/jats:p>","DOI":"10.1128\/iai.01244-06","type":"journal-article","created":{"date-parts":[[2006,11,14]],"date-time":"2006-11-14T02:33:56Z","timestamp":1163471636000},"page":"774-780","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":120,"title":["Characterization of the Urease Operon of\n            <i>Brucella abortus<\/i>\n            and Assessment of Its Role in Virulence of the Bacterium"],"prefix":"10.1128","volume":"75","author":[{"given":"Fe\u0301lix J.","family":"Sangari","sequence":"first","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular, Facultad de Medicina, Universidad de Cantabria, c\/Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuncio\u0301n","family":"Seoane","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular, Facultad de Medicina, Universidad de Cantabria, c\/Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a Cruz","family":"Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular, Facultad de Medicina, Universidad de Cantabria, c\/Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesu\u0301s","family":"Agu\u0308ero","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular, Facultad de Medicina, Universidad de Cantabria, c\/Cardenal Herrera Oria s\/n, 39011 Santander, Spain"},{"name":"Servicio de Microbiologi\u0301a, Hospital Universitario Marque\u0301s de Valdecilla, Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Garci\u0301a Lobo","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular, Facultad de Medicina, Universidad de Cantabria, c\/Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00231-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.22.4305"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1148-1155.2006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1242-1247.2001"},{"key":"e_1_3_2_6_2","first-page":"145","volume":"64","year":"2000","unstructured":"Bosse, J. T., and J. I. MacInnes. 2000. Urease activity may contribute to the ability of Actinobacillus pleuropneumoniae to establish infection. Can. J. Vet. Res.64:145-150.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8353-8361.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.5.1216"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01220.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)31838-1"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.10.3790-3795.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.1.364-367.1993"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01306.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.7.2748-2754.1993"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.2.426-434.2001"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.20.7150-7154.2005"},{"key":"e_1_3_2_20_2","first-page":"800","volume":"16","year":"1994","unstructured":"Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques16:800-802.","journal-title":"BioTechniques"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5060-5066.1998"},{"key":"e_1_3_2_22_2","unstructured":"Madkour M. M. 1989. Brucellosis. Butterworths London. United Kingdom."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2005.06.006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)90957-3"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.6.2036-2042.1991"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.3.451-480.1995"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.5.C333"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192319099"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02533.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1989.tb00262.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4041-4047.1999"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90611-6"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090820"},{"key":"e_1_3_2_34_2","unstructured":"Sambrook J. T. Maniatis and E. F. Fritsch. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Cold Spring Harbor NY."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(91)90040-H"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-13-4-507"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1183-784"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.6.2498-2504.1993"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3253-3258.1993"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408419009105726"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.12.3568-3575.1994"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2005.tb02786.x"},{"key":"e_1_3_2_43_2","first-page":"2053","year":"2000","unstructured":"Young, E. J. 2000. Brucella species, p. 2053-2060. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Mandell, Douglas, and Bennett's principles and practice of infectious diseases, 4th ed. Churchill Livingstone, Philadelphia, PA.","journal-title":"Mandell, Douglas, and Bennett's principles and practice of infectious diseases"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.22.6487-6495.1996"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01244-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01244-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:52:41Z","timestamp":1646463161000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01244-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1128\/IAI.01244-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.01244-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]},"assertion":[{"value":"2006-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}