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We confirm that mucin concentrations are significantly decreased in airway secretions of subjects with cystic fibrosis (CF) who have chronic<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>infection. In sputum from CF subjects without a history of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>, we found no significant difference in the mucin concentration compared to mucus from normal controls. We demonstrate that mucins can be degraded by synthetic human neutrophil elastase (HNE) and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>elastase B (pseudolysin) and that degradation was inhibited by serine proteases inhibitors (diisopropyl fluorophosphates [DFP], phenylmethylsulfonyl fluoride [PMSF], and 1-chloro-3-tosylamido-7-amino-2-heptanone HCl [TLCK]). The mucin concentration in airway secretions from CF subjects is similar to that for normal subjects until there is infection by<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>, and after that, the mucin concentration decreases dramatically. This is most likely due to degradation by serine proteases. The loss of this mucin barrier may contribute to chronic airway infection in the CF airway.<\/jats:p>","DOI":"10.1128\/iai.01252-10","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T04:40:50Z","timestamp":1307421650000},"page":"3438-3444","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Serine Proteases Degrade Airway Mucins in Cystic Fibrosis"],"prefix":"10.1128","volume":"79","author":[{"given":"Markus O.","family":"Henke","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine, Philipps-University Marburg, Baldingerstrasse 1, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit","family":"John","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Philipps-University Marburg, Baldingerstrasse 1, 35043 Marburg, Germany"},{"name":"Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum M\u00fcnchen, Ingolst\u00e4dter Landstra\u00dfe 1, 85764 Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Rheineck","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Philipps-University Marburg, Baldingerstrasse 1, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashi","family":"Chillappagari","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Philipps-University Marburg, Baldingerstrasse 1, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Naehrlich","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Justus-Liebig-Universit\u00e4t Giessen, Feulgenstrasse 12, 35385 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce K.","family":"Rubin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1001 East Marshall Street, Richmond, Virginia 23298"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400796200"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(06)79005-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.10.5565-5575.2003"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/352070a0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0598-615"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000196490"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.150.1.7912987"},{"key":"e_1_3_3_9_2","first-page":"3067","article-title":"Biochemistry of airway mucus secretions","volume":"39","author":"Boat T. 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