{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T01:05:37Z","timestamp":1793667937175,"version":"4.5.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The type VI secretion system (T6SS) is recognized as an important virulence mechanism in several Gram-negative pathogens. In\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n                    , the causative agent of the diarrheal disease cholera, a minimum of three gene clusters\u2014one main cluster and two auxiliary clusters\u2014are required to form a functional T6SS apparatus capable of conferring virulence toward eukaryotic and prokaryotic hosts. Despite an increasing understanding of the components that make up the T6SS apparatus, little is known about the regulation of these genes and the gene products delivered by this nanomachine. VasH is an important regulator of the\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n                    T6SS. Here, we present evidence that VasH regulates the production of a newly identified protein, VasX, which in turn requires a functional T6SS for secretion. Deletion of\n                    <jats:italic>vasX<\/jats:italic>\n                    does not affect export or enzymatic function of the structural T6SS proteins Hcp and VgrG-1, suggesting that VasX is dispensable for the assembly of the physical translocon complex. VasX localizes to the bacterial membrane and interacts with membrane lipids. We present VasX as a novel virulence factor of the T6SS, as a\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n                    mutant lacking\n                    <jats:italic>vasX<\/jats:italic>\n                    exhibits a phenotype of attenuated virulence toward\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Dictyostelium discoideum.<\/jats:named-content>\n                  <\/jats:p>","DOI":"10.1128\/iai.01266-10","type":"journal-article","created":{"date-parts":[[2011,5,10]],"date-time":"2011-05-10T01:59:38Z","timestamp":1304992778000},"page":"2941-2949","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":217,"title":["Vibrio cholerae Requires the Type VI Secretion System Virulence Factor VasX To Kill Dictyostelium discoideum"],"prefix":"10.1128","volume":"79","author":[{"given":"Sarah T.","family":"Miyata","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Kitaoka","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa M.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"McAuley","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Pukatzki","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 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