{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:05:48Z","timestamp":1791219948879,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Intracellular\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium (serovar Typhimurium) occupies a\n            <jats:italic>Salmonella<\/jats:italic>\n            -containing vacuole (SCV) where bacterial effector proteins are secreted into the host cell using type III secretion systems (T3SS). Cytoskeletal motor proteins and T3SS-delivered effector proteins facilitate SCV positioning to juxtanuclear positions where bacterial replication occurs. Here, we show that this characteristic SCV positioning is not maintained by all SCVs during infection of HeLa cells. Notably, juxtanuclear SCV localization that occurs by 8 to 14 h postinfection is followed by significant centrifugal displacement of a subset of SCVs toward the host cell periphery by 24 h postinfection. This novel phenotype requires bacterial protein synthesis, a functional\n            <jats:italic>Salmonella<\/jats:italic>\n            pathogenicity island 2 (SPI-2)-encoded T3SS, intact microtubules, and kinesin-1 motor protein. Bacteria lacking PipB2, a kinesin-recruiting T3SS effector, did not exhibit centrifugal displacement and remained at juxtanuclear positions throughout 24 h of infection. While levels of the SPI-2 effectors PipB2 and SifA increased during 24 h postinfection, a corresponding decrease in levels of the SPI-1 T3SS effectors SipA and SopB, both known to mediate juxtanuclear SCV positioning, was observed. A fluorescence-based assay indicated that wild-type serovar Typhimurium transferred from infected to uninfected epithelial cells while strains deficient in SPI-2 T3SS secretion or PipB2 did not. Our results reveal a novel SCV phenotype implicated in the cell-to-cell spread of serovar Typhimurium during infection.\n          <\/jats:p>","DOI":"10.1128\/iai.01275-08","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T01:45:28Z","timestamp":1229996728000},"page":"996-1007","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["<i>Salmonella<\/i>\n            -Containing Vacuoles Display Centrifugal Movement Associated with Cell-to-Cell Transfer in Epithelial Cells"],"prefix":"10.1128","volume":"77","author":[{"given":"Jason","family":"Szeto","sequence":"first","affiliation":[{"name":"Program in Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Namolovan","sequence":"additional","affiliation":[{"name":"Program in Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne E.","family":"Osborne","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian K.","family":"Coombes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Brumell","sequence":"additional","affiliation":[{"name":"Program in Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada"},{"name":"Department of Molecular Genetics and Institute of Medical Science, University of Toronto, Toronto, Ontario M5S 1A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00706.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00454.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00780.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1204-1208.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110225"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.02.001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28995-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30604.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00087.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01048.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404299200"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00648-06"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00436.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00529.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(91)90013-Z"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707352200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03313.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.53"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10544"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00949"},{"key":"e_1_3_2_23_2","first-page":"131","volume":"289","year":"2005","unstructured":"Guiney, D. G. 2005. The role of host cell death in Salmonella infections. Curr. Top. Microbiol. Immunol.289:131-150.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1788"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-02-0092"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01099.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605443103"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00649.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01047.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1821"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703218104"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04344.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2003.00118.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-04-0367"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03598.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00422.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-08-0614"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2003.12.009"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01651.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7800"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.10.002"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00977.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.569"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-992"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg517"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00137.x"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00898-07"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2593"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00296.x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.1999.00003.x"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02497.x"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1320"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.3954-3962.2005"},{"key":"e_1_3_2_55_2","first-page":"109","volume":"50","year":"1967","unstructured":"Takeuchi, A. 1967. Electron microscope studies of experimental Salmonella infection. I. Penetration into the intestinal epithelium by Salmonella typhimurium. Am. J. Pathol.50:109-136.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4143-1_28"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90366-J"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00152-08"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00294.x"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01275-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01275-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:52:25Z","timestamp":1646466745000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01275-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1128\/IAI.01275-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.01275-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]},"assertion":[{"value":"2008-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}