{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:31:11Z","timestamp":1790386271602,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Ontario Graduate Scholarship"},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Gouvernement du Canada | Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003400","name":"Ministry of Research and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003400","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nasopharyngeal colonization by the Gram-positive bacterium<jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>is a prerequisite for pneumonia and invasive pneumococcal diseases. Colonization is asymptomatic, involving dynamic and complex interplay between commensals, the host immune system, and environmental factors. The elderly are at an increased risk of developing pneumonia, which might be due to changes in the respiratory microbiota that would impact bacterial colonization and persistence within this niche. We hypothesized that the composition of the upper respiratory tract (URT) microbiota changes with age and subsequently can contribute to sustained colonization and inefficient clearance of<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>. To test this, we used a mouse model of pneumococcal colonization to compare the composition of the URT microbiota in young, middle-aged, and old mice in the naive state and during the course of colonization using nasal pharyngeal washes. Sequencing of variable region 3 (V3) of the 16S rRNA gene was used to identify changes occurring with age and throughout the course of<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>colonization. We discovered that age affects the composition of the URT microbiota and that colonization with<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>is more disruptive of preexisting communities in older mice. We have further shown that host-pathogen interactions following<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>colonization can impact the populations of resident microbes, including<jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Haemophilus<\/jats:named-content>. Together, our findings indicate alterations to the URT microbiota could be detrimental to the elderly, resulting in increased colonization of<jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>and decreased efficiency in its clearance.<\/jats:p>","DOI":"10.1128\/iai.01275-15","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T03:58:49Z","timestamp":1453262329000},"page":"906-916","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Streptococcus pneumoniae Colonization Disrupts the Microbial Community within the Upper Respiratory Tract of Aging Mice"],"prefix":"10.1128","volume":"84","author":[{"given":"Netusha","family":"Thevaranjan","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada"},{"name":"McMaster Immunology Research Centre, McMaster University, Hamilton, Canada"},{"name":"Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona J.","family":"Whelan","sequence":"additional","affiliation":[{"name":"Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Canada"},{"name":"Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Puchta","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada"},{"name":"McMaster Immunology Research Centre, McMaster University, Hamilton, Canada"},{"name":"Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eta","family":"Ashu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada"},{"name":"McMaster Immunology Research Centre, McMaster University, Hamilton, Canada"},{"name":"Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rossi","sequence":"additional","affiliation":[{"name":"Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Surette","sequence":"additional","affiliation":[{"name":"Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada"},{"name":"Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Canada"},{"name":"Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada"},{"name":"Department of Medicine, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn M. E.","family":"Bowdish","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada"},{"name":"McMaster Immunology Research Centre, McMaster University, Hamilton, Canada"},{"name":"Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00618-13"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-146779"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/381547"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000000335"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.4974-4976.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086136"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31814da70c"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201310-351OC"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090974"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199806253382601"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119875"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01546-10"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2217\/ahe.09.74"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00931-4"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis240"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003057"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.6.716"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-59"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-483"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00317-06"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1044-6"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/500942"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318270d850"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12073"},{"key":"e_1_3_3_26_2","first-page":"e50490","article-title":"Characterization of inflammatory responses during intranasal colonization with Streptococcus pneumoniae","volume":"2014","author":"Puchta A","year":"2014","unstructured":"Puchta A, Verschoor CP, Thurn T, Bowdish DME. 2014. Characterization of inflammatory responses during intranasal colonization with Streptococcus pneumoniae. J Vis Exp 2014:e50490.","journal-title":"J Vis Exp"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005368"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005004"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202113"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.250"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02772-10"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-31"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1109\/BIBM.2010.5706555"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02498-06"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023281"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003531"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109710"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.03.073"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91434-X"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15021-0"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.124776"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.09.049"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ096"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017035"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208075109"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.05.005"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.05.051"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3391\/mbi.2013.4.1.01"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0010001"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.130224"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-2-44"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2014.06.001"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00129-10"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306398101"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2540"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2259"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks348"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11319"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000097107"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01290-13"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.294.16.2043"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.3.2005075"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.164.3.2008149"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1086\/600870"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00147-14"},{"key":"e_1_3_3_69_2","doi-asserted-by":"crossref","first-page":"2750","DOI":"10.4049\/jimmunol.147.8.2750","article-title":"Repertoire diversity of antibody response to bacterial antigens in aged mice. II. Phosphorylcholine-antibody in young and aged mice differ in both VH\/VL gene repertoire and in specificity","volume":"147","author":"Nicoletti C","year":"1991","unstructured":"Nicoletti C, Borghesi-Nicoletti C, Yang XH, Schulze DH, Cerny J. 1991. Repertoire diversity of antibody response to bacterial antigens in aged mice. II. Phosphorylcholine-antibody in young and aged mice differ in both VH\/VL gene repertoire and in specificity. J Immunol 147:2750\u20132755.","journal-title":"J Immunol"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01275-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01275-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T15:02:53Z","timestamp":1692198173000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01275-15"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":68,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1128\/IAI.01275-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.01275-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"2015-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}