{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:48:08Z","timestamp":1784612888514,"version":"3.55.0"},"reference-count":73,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI091957"],"award-info":[{"award-number":["AI091957"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            , the causative agent of the severe diarrheal disease cholera, thrives in both marine environments and the human host. To do so, it must encode the tools necessary to acquire essential nutrients, including iron, under these vastly different conditions. A number of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            iron acquisition systems have been identified; however, the precise role of each system is not fully understood. To test the roles of individual systems, we generated a series of mutants in which only one of the four systems that support iron acquisition on unsupplemented LB agar, Feo, Fbp, Vct, and Vib, remains functional. Analysis of these mutants under different growth conditions showed that these systems are not redundant. The strain carrying only the ferrous iron transporter Feo grew well at acidic, but not alkaline, pH, whereas the ferric iron transporter Fbp promoted better growth at alkaline than at acidic pH. A strain defective in all four systems (null mutant) had a severe growth defect under aerobic conditions but accumulated iron and grew as well as the wild type in the absence of oxygen, suggesting the presence of an additional, unidentified iron transporter in\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            . In support of this, the null mutant was only moderately attenuated in an infant mouse model of infection. While the null mutant used heme as an iron source\n            <jats:italic>in vitro<\/jats:italic>\n            , we demonstrate that heme is not available to\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            in the infant mouse intestine.\n          <\/jats:p>","DOI":"10.1128\/iai.01301-15","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T15:05:36Z","timestamp":1449587136000},"page":"511-523","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Nonredundant Roles of Iron Acquisition Systems in Vibrio cholerae"],"prefix":"10.1128","volume":"84","author":[{"given":"Eric D.","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth E.","family":"Wyckoff","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra R.","family":"Mey","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn R.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley M.","family":"Payne","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas, Austin, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/416359"},{"key":"e_1_3_2_3_2","first-page":"17","article-title":"Chemical factors that influence the availability of iron and manganese in aqueous systems","volume":"140","author":"Hem JD","year":"1972","unstructured":"Hem JD. 1972. Chemical factors that influence the availability of iron and manganese in aqueous systems. Geol Soc Am Spec Pap 140:17\u201324.","journal-title":"Geol Soc Am Spec Pap"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1991.36.8.1793"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.06.007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00080"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.04.010"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12087"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.20.1.310-311.1978"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43671-4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00626-06"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07775.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-006-9073-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.7055-7062.1997"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi001651a"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0016523"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.11.3729-3738.1992"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.10.3270-3274.1992"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01034.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.24.7588-7596.1999"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3419-3426.2002"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.18.5631-5638.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01137.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.11.3269-3277.1994"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02683.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.19.6212-6219.1993"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00738-13"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.7.3.445-456.1973"},{"key":"e_1_3_2_29_2","first-page":"831","article-title":"Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis","volume":"15","author":"Schweizer HD","year":"1993","unstructured":"Schweizer HD. 1993. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. Biotechniques 15:831\u2013834.","journal-title":"Biotechniques"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.2833"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.29.8.1035"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781118349083"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8167-8178.2005"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00391a039"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107894108"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1991.tb04617.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.12.3151-3158.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002342107"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.140"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5706-5719.2005"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01721-2"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.96.2.570-572.1968"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.6.2919-2924.1989"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.016"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4MT00332B"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00086-12"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06333.x"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4MT00333K"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00417-15"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00569-08"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2014.01.007"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12709"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1919-1928.2003"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00562-06"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1135-1147.1998"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01360.x"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05802.x"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.19.6201-6204.1997"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.222745"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.1.156-163.1981"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12003"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.47.2.360-362.1985"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.11.5120-5125.1994"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.07.007"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003800"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1830-1834.2001"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.6.1731-1738.2000"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.8.2350-2353.2000"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/306551a0"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1038\/35020000"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.170.6.2575-2583.1988"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90366-J"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1415-1423.1999"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01301-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.01301-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:10:26Z","timestamp":1646475026000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.01301-15"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":73,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1128\/IAI.01301-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.01301-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2]]},"assertion":[{"value":"2015-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}