{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:37:28Z","timestamp":1789771048371,"version":"4.0.1"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100012308","name":"Natalie V. Zucker Fellowship","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012308","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI034937"],"award-info":[{"award-number":["AI034937"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            is an intracellular bacterial pathogen that replicates in alveolar macrophages, causing a severe form of pneumonia. Intracellular growth of the bacterium depends on its ability to sequester iron from the host cell. In the\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            strain 130b, one mechanism used to acquire this essential nutrient is the siderophore legiobactin. Iron-bound legiobactin is imported by the transport protein LbtU. Here, we describe the role of LbtP, a paralog of LbtU, in iron acquisition in the\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            strain Philadelphia-1. Similar to LbtU, LbtP is a siderophore transport protein and is required for robust growth under iron-limiting conditions. Despite their similar functions, however, LbtU and LbtP do not contribute equally to iron acquisition. The Philadelphia-1 strain lacking LbtP is more sensitive to iron deprivation\n            <jats:italic>in vitro<\/jats:italic>\n            . Moreover, LbtP is important for\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            growth within macrophages while LbtU is dispensable. These results demonstrate that LbtP plays a dominant role over LbtU in iron acquisition. In contrast, loss of both LbtP and LbtU does not impair\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            growth in the amoebal host\n            <jats:named-content content-type=\"genus-species\">Acanthamoeba castellanii<\/jats:named-content>\n            , demonstrating a host-specific requirement for the activities of these two transporters in iron acquisition. The growth defect of the \u0394\n            <jats:italic>lbtP<\/jats:italic>\n            mutant in macrophages is not due to alterations in growth kinetics. Instead, the absence of LbtP limits\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            replication and causes bacteria to prematurely exit the host cell. These results demonstrate the existence of a preprogrammed exit strategy in response to iron limitation that allows\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            to abandon the host cell when nutrients are exhausted.\n          <\/jats:p>","DOI":"10.1128\/iai.01306-15","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T02:29:30Z","timestamp":1463452170000},"page":"2185-2197","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Iron Limitation Triggers Early Egress by the Intracellular Bacterial Pathogen Legionella pneumophila"],"prefix":"10.1128","volume":"84","author":[{"given":"Tamara J.","family":"O'Connor","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaixin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"VanRheenen","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soma","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P.","family":"Cianciotto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph R.","family":"Isberg","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts, USA"},{"name":"Howard Hughes Medical Institute, Chevy Chase, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.41.1.9-16.1981"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1387-2656(05)11011-4"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.12.1179"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/498575"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109874"},{"key":"e_1_3_3_8_2","first-page":"32","article-title":"Community-acquired Legionnaires' disease","volume":"4","author":"Muder RR","year":"1989","unstructured":"Muder RR, Yu VL, Fang G-D. 1989. 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