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We analyzed immune responses in<jats:italic>Helicobacter<\/jats:italic>-infected, normal mice depleted of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>T cells to investigate the in vivo role of regulatory T cells (Tregs) in the modulation of<jats:italic>Helicobacter<\/jats:italic>immunopathology. BALB\/c and transgenic mice were depleted of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>T cells by administration of an anti-CD25 antibody either at the time of infection with<jats:italic>Helicobacter<\/jats:italic>or during chronic infection and gastritis. Depletion of CD25<jats:sup>+<\/jats:sup>Tregs prior to and during infection of mice with<jats:italic>Helicobacter<\/jats:italic>spp. did not affect either bacterial colonization or severity of gastritis. Depletion of CD25<jats:sup>+<\/jats:sup>Tregs was associated with increased<jats:italic>Helicobacter<\/jats:italic>-specific antibody levels and an altered isotype distribution. Paragastric lymph node cells from CD25<jats:sup>+<\/jats:sup>Treg-depleted and control infected mice showed similar proliferation to<jats:italic>Helicobacter<\/jats:italic>antigens, but only cells from anti-CD25-treated animals secreted Th2 cytokines. CD25<jats:sup>+<\/jats:sup>Tregs do not control the level of gastritis induced by gastric<jats:italic>Helicobacter<\/jats:italic>spp. in normal, thymus-intact BALB\/c mice. However, CD25<jats:sup>+<\/jats:sup>Tregs influence the cytokine and antibody responses induced by infection. Autoimmune gastritis is not induced in<jats:italic>Helicobacter<\/jats:italic>-infected mice depleted of CD25<jats:sup>+<\/jats:sup>Tregs but is induced in CD25<jats:sup>+<\/jats:sup>Treg-depleted mice, which have a higher frequency of autoreactive T cells.<\/jats:p>","DOI":"10.1128\/iai.01314-05","type":"journal-article","created":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T23:52:41Z","timestamp":1148082761000},"page":"3519-3529","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["CD4<sup>+<\/sup>CD25<sup>+<\/sup>Regulatory T Cells Modulate the T-Cell and Antibody Responses in<i>Helicobacter<\/i>-Infected BALB\/c Mice"],"prefix":"10.1128","volume":"74","author":[{"given":"Maria","family":"Kaparakis","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology and the NHMRC Bacterial Pathogenesis Group, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen L.","family":"Laurie","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odilia","family":"Wijburg","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology and the NHMRC Bacterial Pathogenesis Group, The University of Melbourne, Parkville, Victoria 3010, Australia"},{"name":"CRC for Vaccine Technology, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Pearse","sequence":"additional","affiliation":[{"name":"CSL Ltd., Poplar Rd., Parkville, Victoria 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"van Driel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Gleeson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Strugnell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology and the NHMRC Bacterial Pathogenesis Group, The University of Melbourne, Parkville, Victoria 3010, Australia"},{"name":"CRC for Vaccine Technology, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.8.5024"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/12.3.343"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.9.4850"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030530"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.2.387"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.6.3232"},{"key":"e_1_3_2_8_2","first-page":"204","volume":"68","year":"1989","unstructured":"Asensi, V., K. 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