{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:54:49Z","timestamp":1790902489585,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n            , the causative agent of scrub typhus, is an obligate intracellular pathogen. Previously, we reported that the 56-kDa type-specific antigen (TSA56), a major outer membrane protein of\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            , binds to fibronectin and facilitates bacterial entry into the host cell, potentially via an interaction with integrins. Here, we demonstrated that\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            colocalizes with integrin \u03b15\u03b21 and activates integrin signaling effectors, including focal adhesion kinase, Src kinase, and RhoA GTPase, and also recruits signaling adaptors, such as talin and paxillin, to the site of infection. Inhibition of protein tyrosine kinases or RhoA reduced intracellular invasion. We also observed substantial actin reorganization and membrane protrusions at the sites of infection of nonphagocytic host cells. Finally, we identified a region in the extracellular domain of TSA56 that binds to fibronectin. A peptide containing this region was able to significantly reduce bacterial invasion. Taken together, these results clearly indicate that\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            exploits integrin-mediated signaling and the actin cytoskeleton for invasion of eukaryotic host cells.\n          <\/jats:p>","DOI":"10.1128\/iai.01316-09","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T04:34:18Z","timestamp":1266381258000},"page":"1915-1923","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Intracellular Invasion by\n            <i>Orientia tsutsugamushi<\/i>\n            Is Mediated by Integrin Signaling and Actin Cytoskeleton Rearrangements"],"prefix":"10.1128","volume":"78","author":[{"given":"Bon-A","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine, Jongno-Gu, Seoul 110-799, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam-Hyuk","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine, Jongno-Gu, Seoul 110-799, Republic of Korea"},{"name":"Institute of Endemic Disease, Seoul National University Medical Research Center and Bundang Hospital, Jongno-Gu, Seoul 110-799, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Yong","family":"Seong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine, Jongno-Gu, Seoul 110-799, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung-Sik","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine, Jongno-Gu, Seoul 110-799, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ik-Sang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine, Jongno-Gu, Seoul 110-799, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00768.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00201-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.3.3.4009"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611553104"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.594-602.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.018036"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2846-07.2007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309693200"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5438.291"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.12.002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/596576"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2007.22.1.1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04744.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/589284"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.6.1973"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-82"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01382"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.08.046"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07391.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2006.08.011"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00768.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.003566"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.012"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90163-7"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.06.002"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01352-6"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03222.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)02501-9"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200310000-00009"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200508075"},{"key":"e_1_3_2_32_2","first-page":"338","volume":"114","year":"2009","unstructured":"Xu, 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Li, G. D. Ferguson, F. Mercurio, G. Khambatta, L. Morrison, A. Lopez-Girona, L. G. Corral, D. R. Webb, B. L. Bennett, and W. Xie. 2009. Immunomodulatory drugs reorganize cytoskeleton by modulating Rho GTPases. 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