{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T06:29:43Z","timestamp":1769581783148,"version":"3.49.0"},"reference-count":82,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycoplasma genitalium<\/jats:named-content>\n            is a sexually transmitted pathogen associated with several acute and chronic reproductive tract disease syndromes in men and women. To evaluate the suitability of a pig-tailed macaque model of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. genitalium<\/jats:named-content>\n            infection, we inoculated a pilot animal with\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. genitalium<\/jats:named-content>\n            strain G37 in the uterine cervix and in salpingeal pockets generated by transplanting autologous Fallopian tube tissue subcutaneously. Viable organisms were recovered throughout the 8-week experiment in cervicovaginal specimens and up to 2 weeks postinfection in salpingeal pockets. Humoral and cervicovaginal antibodies reacting to MgpB were induced postinoculation and persisted throughout the infection. The immunodominance of the MgpB adhesin and the accumulation of\n            <jats:italic>mgpB<\/jats:italic>\n            sequence diversity previously observed in persistent human infections prompted us to evaluate sequence variation in this animal model. We found that after 8 weeks of infection, sequences within\n            <jats:italic>mgpB<\/jats:italic>\n            variable region B were replaced by novel sequences generated by reciprocal recombination with an archived variant sequence located elsewhere on the chromosome. In contrast,\n            <jats:italic>mgpB<\/jats:italic>\n            region B of the same inoculum propagated for 8 weeks\n            <jats:italic>in vitro<\/jats:italic>\n            remained unchanged. Notably, serum IgG reacted strongly with a recombinant protein spanning MgpB region B of the inoculum, while reactivity to a recombinant protein representing the week 8 variant was reduced, suggesting that antibodies were involved in the clearance of bacteria expressing the original infecting sequence. Together these results suggest that the pig-tailed macaque is a suitable model to study\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. genitalium<\/jats:named-content>\n            pathogenesis, antibody-mediated selection of antigenic variants\n            <jats:italic>in vivo<\/jats:italic>\n            , and immune escape.\n          <\/jats:p>","DOI":"10.1128\/iai.01322-12","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T00:21:25Z","timestamp":1370305285000},"page":"2938-2951","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Persistence, Immune Response, and Antigenic Variation of Mycoplasma genitalium in an Experimentally Infected Pig-Tailed Macaque (Macaca nemestrina)"],"prefix":"10.1128","volume":"81","author":[{"given":"Gwendolyn E.","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie L.","family":"Iverson-Cabral","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy L.","family":"Patton","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter K.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne T.","family":"Cosgrove Sweeney","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A.","family":"Totten","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Seattle, Washington, USA"},{"name":"Global Health, Pathobiology Interdisciplinary Program, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir702"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001324"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)91411-E"},{"key":"e_1_3_2_6_2","first-page":"265","article-title":"Mycoplasma genitalium: a cause of male urethritis?","volume":"69","author":"Jensen JS","year":"1993","unstructured":"JensenJSOrsumRDohnBUldumSWormAMLindK. 1993. 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